Determination of the composition and the allelic state of disease and pest resistance genes in potato parental lines using DNA markers

Abstract

The allelic dosage of disease and pest resistance genes was determined in 11 prospective potato varieties and hybrids by means of detecting the corresponding PCR DNA markers in their progeny from crosses with specially selected testers. It was revealed that most (65%) resistance genes in the analyzed parental lines were present as a single dominant allele (simplex). Nevertheless, we were able to find some multiplex lines valuable for breeding. The Yanka variety and the clone 52-03-16 had one triplex and one duplex of resistance genes, the Lilea and Charaunik varieties and the clone 106-04-17 had two resistance genes in duplex, and the Uladar and Falvarak varieties and the clone 45-04-24 were duplex for some single genes. The highest number of multiplex lines was detected for the genes Ry_sto, H1, and Sen1. Only simplex genotypes were revealed for the Gro-1-4 and PLRV1 genes.