Email this article Features of expression of the PsSst1 and PsIgn1 genes in nodules of pea (Pisum sativum L.) symbiotic mutants
- Authors: Zhukov V.A.1, Rychagov T.S.1, Fedorina J.V.1, Pinaev A.G.1, Andronov E.E.1, Borisov A.Y.1, Tikhonovich I.A.1,2
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Affiliations:
- All-Russia Research Institute for Agricultural Microbiology
- Department of Genetics and Biotechnology
- Issue: Vol 52, No 4 (2016)
- Pages: 362-369
- Section: Genetics of Microorganisms
- URL: https://journals.rcsi.science/1022-7954/article/view/187674
- DOI: https://doi.org/10.1134/S1022795416040128
- ID: 187674
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Abstract
The sequences of the PsSst1 and PsIgn1 genes of pea (Pisum sativum L.) homologous to the symbiotic LjSST1 and LjIGN1 genes of Lotus japonicus (Regel.) K. Larsen are determined. The expression level of PsSst1 and PsIgn1 genes is determined by real-time PCR in nodules of several symbiotic mutants and original lines of pea. Lines with increased (Sprint-2Fix– (Pssym31)) and decreased (P61 (Pssym25)) expression level of both genes are revealed along with the lines characterized by changes in the expression level of only one of these genes. The revealed features of the PsSst1 and PsIgn1 expression allow us to expand the phenotypic characterization of pea symbiotic mutants. In addition, PsSst1 and PsIgn1 cDNA is sequenced in selected mutant lines, characterized by a decreased expression level of these genes in nodules, but no mutations are found.
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About the authors
V. A. Zhukov
All-Russia Research Institute for Agricultural Microbiology
Author for correspondence.
Email: vladimir.zhukoff@gmail.com
Russian Federation, St. Petersburg, Pushkin, 196608
T. S. Rychagov
All-Russia Research Institute for Agricultural Microbiology
Email: vladimir.zhukoff@gmail.com
Russian Federation, St. Petersburg, Pushkin, 196608
J. V. Fedorina
All-Russia Research Institute for Agricultural Microbiology
Email: vladimir.zhukoff@gmail.com
Russian Federation, St. Petersburg, Pushkin, 196608
A. G. Pinaev
All-Russia Research Institute for Agricultural Microbiology
Email: vladimir.zhukoff@gmail.com
Russian Federation, St. Petersburg, Pushkin, 196608
E. E. Andronov
All-Russia Research Institute for Agricultural Microbiology
Email: vladimir.zhukoff@gmail.com
Russian Federation, St. Petersburg, Pushkin, 196608
A. Yu. Borisov
All-Russia Research Institute for Agricultural Microbiology
Email: vladimir.zhukoff@gmail.com
Russian Federation, St. Petersburg, Pushkin, 196608
I. A. Tikhonovich
All-Russia Research Institute for Agricultural Microbiology; Department of Genetics and Biotechnology
Email: vladimir.zhukoff@gmail.com
Russian Federation, St. Petersburg, Pushkin, 196608; St. Petersburg, 199034
