A comparative analysis of methylation status of tumor suppressor genes in paired biopsy and serum samples from cervical cancer patients among north indian population
- Authors: Jha A.K.1, Sharma V.1, Nikbakht M.1, Jain V.2, Sehgal A.2, Capalash N.1, Kaur J.1
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Affiliations:
- Department of Biotechnology
- Department of Gynaecology
- Issue: Vol 52, No 2 (2016)
- Pages: 226-230
- Section: Human Genetics
- URL: https://journals.rcsi.science/1022-7954/article/view/187648
- DOI: https://doi.org/10.1134/S1022795416010075
- ID: 187648
Cite item
Abstract
Tumor-specific genetic or epigenetic alterations have been detected in serum DNA in case of various types of cancers. In breast cancer, the detection of tumor suppressor gene hypermethylation has been reported in several body fluids. Promoter hypermethylation of some genes like MYOD1, CALCA, hTERT, etc. has also been detected in serum samples from cervical cancer. The present study is the first report on the comparison of promoter hypermethylation of tumor suppressor genes like p14, p15, p16, p21, p27, p57, p53, p73, RARβ2, FHIT, DAPK, STAT1, and RB1 genes in paired biopsy and serum samples from cervical cancer patients among north Indian population. This is also the first report on the hypermethylation of these genes in serum samples from cervical cancer patients among north Indian population. According to the results of the present study, promoter hypermethylation of these genes can also be detected in serum samples of cervical cancer patients. The sensitivity of detection of promoter hypermethylalion in serum samples of cervical cancer patients as compared to paired biopsy samples was found to be around 83.3%. It was observed that promoter hypermethylation was mainly observed in the serum samples in the higher stages and very rarely in the lower stages. The present study clearly showed that serum of patients with cervical cancer can also be used to study methylated genes as biomarkers.
Keywords
About the authors
A. K. Jha
Department of Biotechnology; Department of Biotechnology
Author for correspondence.
Email: jagsekhon@yahoo.com
India, Chandigarh, 160014; Ghaziabad, 201009
V. Sharma
Department of Biotechnology
Email: jagsekhon@yahoo.com
India, Chandigarh, 160014
M. Nikbakht
Department of Biotechnology
Email: jagsekhon@yahoo.com
India, Chandigarh, 160014
V. Jain
Department of Gynaecology
Email: jagsekhon@yahoo.com
India, Ludhiana
A. Sehgal
Department of Gynaecology
Email: jagsekhon@yahoo.com
India, Chandigarh
N. Capalash
Department of Biotechnology
Email: jagsekhon@yahoo.com
India, Chandigarh, 160014
J. Kaur
Department of Biotechnology
Email: jagsekhon@yahoo.com
India, Chandigarh, 160014