Proteomic Analysis of Kunitz-Type Trypsin Inhibitor Deleted Soybean


Дәйексөз келтіру

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Аннотация

To explore proteomic characters of Kunitz-type trypsin inhibitors (KTIs) deleted soybean (Glycine max (L.) Merr.), seeds without KTIs and its female parent with KTIs were analyzed for differentially expressed proteins and specially expressed proteins using a label-free quantitative approach. A total of 1093 proteins were identified, with 139 up-regulated proteins among 169 differentially expressed proteins in both. Functional classification revealed that differentially expressed proteins were involved in almost functional categories, and carbohydrate metabolic process was the most abundant by enrichment analysis. Pathway analysis indicated that the differentially expressed proteins were mainly enriched into ribosome pathway, then carbohydrate metabolism. KTI3 (trypsin inhibitors A) interacted with β-conglycinin was independent from a complex protein network by proteins interactions analysis. Expression of KTI3 decreased in the seed of KTIs deleted soybean, which made for protein biosynthetic processes, metabolic processes and resistance processes. The specially expressed proteins analysis provided new information for the proteomic metabolism of KTIs deleted soybean resource during seed development, but it need further investigate for unknown information of KTIs deleted soybean.

Авторлар туралы

Y. Jiang

College of Agriculture, Northeast Agricultural University; Heilongjiang Green Good Science Institute, Northeast Agricultural University

Email: cgw603@163.com
ҚХР, Harbin, 150030; Harbin, Heilongjiang, 150028

Y. Li

College of Agriculture, Northeast Agricultural University; Heilongjiang Green Good Science Institute, Northeast Agricultural University

Email: cgw603@163.com
ҚХР, Harbin, 150030; Harbin, Heilongjiang, 150028

S. Wang

College of Agriculture, Northeast Agricultural University; Heilongjiang Green Good Science Institute, Northeast Agricultural University

Хат алмасуға жауапты Автор.
Email: wsdhlj@aliyun.com
ҚХР, Harbin, 150030; Harbin, Heilongjiang, 150028

G. Cui

Animal Science and Technology College, Northeast Agricultural University

Хат алмасуға жауапты Автор.
Email: cgw603@163.com
ҚХР, Harbin, Heilongjiang, 150030

H. Wang

Heilongjiang Green Good Science Institute, Northeast Agricultural University; College of Life Science, Northeast Agricultural University

Email: cgw603@163.com
ҚХР, Harbin, Heilongjiang, 150028; Harbin, Heilongjiang, 150030

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© Pleiades Publishing, Ltd., 2019