Email this article Reduced GA biosynthesis in GmRAV-transgenic tobacco causes the dwarf phenotype
- Authors: Zhao L.1, Yang X.1, Du H.L.1, Li M.M.1, Ding F.Q.1, Lv Q.X.1, Wang C.1, Wang P.P.1, Zhang K.X.1, Nie T.K.1, Li W.B.1
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Affiliations:
- Key Laboratory of Soybean Biology of Chinese Education Ministry (Key Laboratory of Biology and Genetics and Breeding for Soybean in Northeast China, Ministry of Agriculture)
- Issue: Vol 63, No 5 (2016)
- Pages: 690-694
- Section: Research Papers
- URL: https://journals.rcsi.science/1021-4437/article/view/179318
- DOI: https://doi.org/10.1134/S1021443716050198
- ID: 179318
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Abstract
GmRAV-transgenic tobacco plants exhibited decreased gibberellins (GAs) biosynthesis related phenotypes, including dwarfism and repressed flowering time. The dwarf phenotype of GmRAV-overexpressing (ox) tobaccos was fully restored in stem and hypocotyl length of transgenic plants by the application of exogenous GA3. The epidermal cells in the stem internodes of the GmRAV-ox tobacco plants treated with GA3 were obviously the same as those in wild-type plants in longitudinal direction. Therefore, the rescued plant height of GmRAV-ox tobaccos by GA3 might be largely caused by increased cell size. GmRAV overexpression inhibited GA biosynthesis due to a transcriptional repression of GA20ox gene. The dwarfed phenotype of GmRAV-ox tobaccos with reduced stem elongation could be caused by a reduction in GA3 content.
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About the authors
L. Zhao
Key Laboratory of Soybean Biology of Chinese Education Ministry (Key Laboratory of Biology and Genetics and Breeding for Soybean in Northeast China, Ministry of Agriculture)
Email: wenbinli@yahoo.com
China, Harbin
X. Yang
Key Laboratory of Soybean Biology of Chinese Education Ministry (Key Laboratory of Biology and Genetics and Breeding for Soybean in Northeast China, Ministry of Agriculture)
Email: wenbinli@yahoo.com
China, Harbin
H. L. Du
Key Laboratory of Soybean Biology of Chinese Education Ministry (Key Laboratory of Biology and Genetics and Breeding for Soybean in Northeast China, Ministry of Agriculture)
Email: wenbinli@yahoo.com
China, Harbin
M. M. Li
Key Laboratory of Soybean Biology of Chinese Education Ministry (Key Laboratory of Biology and Genetics and Breeding for Soybean in Northeast China, Ministry of Agriculture)
Email: wenbinli@yahoo.com
China, Harbin
F. Q. Ding
Key Laboratory of Soybean Biology of Chinese Education Ministry (Key Laboratory of Biology and Genetics and Breeding for Soybean in Northeast China, Ministry of Agriculture)
Email: wenbinli@yahoo.com
China, Harbin
Q. X. Lv
Key Laboratory of Soybean Biology of Chinese Education Ministry (Key Laboratory of Biology and Genetics and Breeding for Soybean in Northeast China, Ministry of Agriculture)
Email: wenbinli@yahoo.com
China, Harbin
C. Wang
Key Laboratory of Soybean Biology of Chinese Education Ministry (Key Laboratory of Biology and Genetics and Breeding for Soybean in Northeast China, Ministry of Agriculture)
Email: wenbinli@yahoo.com
China, Harbin
P. P. Wang
Key Laboratory of Soybean Biology of Chinese Education Ministry (Key Laboratory of Biology and Genetics and Breeding for Soybean in Northeast China, Ministry of Agriculture)
Email: wenbinli@yahoo.com
China, Harbin
K. X. Zhang
Key Laboratory of Soybean Biology of Chinese Education Ministry (Key Laboratory of Biology and Genetics and Breeding for Soybean in Northeast China, Ministry of Agriculture)
Email: wenbinli@yahoo.com
China, Harbin
T. K. Nie
Key Laboratory of Soybean Biology of Chinese Education Ministry (Key Laboratory of Biology and Genetics and Breeding for Soybean in Northeast China, Ministry of Agriculture)
Email: wenbinli@yahoo.com
China, Harbin
W. B. Li
Key Laboratory of Soybean Biology of Chinese Education Ministry (Key Laboratory of Biology and Genetics and Breeding for Soybean in Northeast China, Ministry of Agriculture)
Author for correspondence.
Email: wenbinli@yahoo.com
China, Harbin
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