Redox Status of a Metastatic Microenvironment in the Liver of Patients with Colorectal Cancer from EPR
- 作者: Burlaka A.1, Vovk A.1, Burlaka A.2, Gafurov M.3, Iskhakova K.3, Ganusevich I.1, Virko S.1,4, Lukin S.1
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隶属关系:
- R.E. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology NAS of Ukraine
- Ukrainian National Cancer Institute
- Kazan Federal University
- V.E. Lashkaryov Institute of Semiconductor Physics NAS of Ukraine
- 期: 卷 50, 编号 1-3 (2019)
- 页面: 391-402
- 栏目: Original Paper
- URL: https://journals.rcsi.science/0937-9347/article/view/248376
- DOI: https://doi.org/10.1007/s00723-018-1093-z
- ID: 248376
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详细
Colorectal cancer is the third most common cancer with the predominant metastases (Mts) into the liver. Metastases, tissues adjacent to Mts (AMT) and remote tissues (RMT, taken at the distance of 5 cm from Mts) from 25 patients with metachronous liver Mts after the liver resection as well as blood and urine were studied by electron paramagnetic resonance (EPR) including the spin-trapping EPR for the detection and quantification of the activity of N2 iron-sulfur proteins, levels of NO-N2 complexes, labile iron pool (LIP), lactoferrin (Lf), superoxide and NO radicals. Activity of metalloproteinase MMP-2 and MMP-9 were determined. In adjacent and remote liver tissues low activity of N2 in mitochondrial electron transport chain (EPR signal with g = 1.94), loss of functions of detoxification system (cytochrome P450, g = 2.25), appearance and growth of NO-N2 complexes (g = 2.007) are obtained. Intensive EPR signals from LIP (g = 2.2–2.4) and Lf (g = 4.3) are registered. Superoxide generation rates in liver tissues and blood are of up to 6 times higher than for the control group (p < 0.001). NO levels are of 1.7 times higher for the AMT compared to RMT (p < 0.05) while being 15 times higher for blood comparing to the reference species (p < 0.001). Activity of MMP-2 and MMP-9 was registered both in AMT and RMT and is in 1.7 times higher in AMT (p < 0.05). The obtained results can be used to estimate the functional state of organs and tissues with distant metastases, the risk of recurrence, to correct the antitumor therapeutic procedures.
作者简介
Anatoly Burlaka
R.E. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology NAS of Ukraine
编辑信件的主要联系方式.
Email: apburlaka@gmail.com
乌克兰, Kiev
Anastasia Vovk
R.E. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology NAS of Ukraine
Email: apburlaka@gmail.com
乌克兰, Kiev
Anton Burlaka
Ukrainian National Cancer Institute
Email: apburlaka@gmail.com
乌克兰, Kiev
Marat Gafurov
Kazan Federal University
Email: apburlaka@gmail.com
俄罗斯联邦, Kazan
Kamila Iskhakova
Kazan Federal University
Email: apburlaka@gmail.com
俄罗斯联邦, Kazan
Irina Ganusevich
R.E. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology NAS of Ukraine
Email: apburlaka@gmail.com
乌克兰, Kiev
Sergei Virko
R.E. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology NAS of Ukraine; V.E. Lashkaryov Institute of Semiconductor Physics NAS of Ukraine
Email: apburlaka@gmail.com
乌克兰, Kiev; Kiev
Sergei Lukin
R.E. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology NAS of Ukraine
Email: apburlaka@gmail.com
乌克兰, Kiev
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