Ovariectomy-Induced Osteoporosis Evaluated by 1H One- and Two-Dimensional NMR Transverse Relaxometry
- Autores: Şipos R.1, Fechete R.2, Moldovan D.2, Şuş I.1, Pávai Z.1, Demco D.2,3
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Afiliações:
- University of Medicine and Pharmacy
- Department of Physics and Chemistry, Technical University of Cluj-Napoca
- DWI-Leibniz Institute for Interactive Materials, RWTH-Aachen University
- Edição: Volume 47, Nº 12 (2016)
- Páginas: 1419-1437
- Seção: Original Paper
- URL: https://journals.rcsi.science/0937-9347/article/view/247603
- DOI: https://doi.org/10.1007/s00723-016-0839-8
- ID: 247603
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Resumo
The aim of this work is to show that the bones with marrow, treated as a quasi-porous media, can be successfully used to study the effects of ovariectomy-induced osteoporosis. Proton one-dimensional (1D) nuclear magnetic resonance (NMR) T2-distribution and two-dimensional (2D) T2–T2 exchange maps combined with histological images were used to measure the proximal part of the femoris, diaphysis and distal epiphysis of ovariectomized and non-ovariectomized Wistar albino rats. The 1D normalized T2 distributions showed four peaks which were associated with protons in four major pools: (1) the protons from bounded water to collagenous matrix; (2) fluids in osteocyte lacunae and canaliculi channels; (3) fluids in secondary pores like Haversian and transverse Volkmann canals and (4) soft matter like bone marrow and fluids in primary pores like trabecular bone cavities. The peak’s association and hierarchical structure of pores in femoral bone were supported by a 2D T2–T2 exchange map and by a series of dehydration experiments monitored by NMR measurements. The bone marrow narrows the T2-distributions, increasing resolution, but will not influence significantly the peaks positions; therefore, the NMR relaxometry is a valuable tool to characterize the pore distributions and effects of induced osteoporosis in diverse bones sections.
Sobre autores
R. Şipos
University of Medicine and Pharmacy
Email: rfechete@phys.utcluj.ro
Romênia, Gh. Marinescu, 38, Tg-Mures, 540139
R. Fechete
Department of Physics and Chemistry, Technical University of Cluj-Napoca
Autor responsável pela correspondência
Email: rfechete@phys.utcluj.ro
Romênia, 28 Memorandumului Street, Cluj-Napoca, 400114
D. Moldovan
Department of Physics and Chemistry, Technical University of Cluj-Napoca
Email: rfechete@phys.utcluj.ro
Romênia, 28 Memorandumului Street, Cluj-Napoca, 400114
I. Şuş
University of Medicine and Pharmacy
Email: rfechete@phys.utcluj.ro
Romênia, Gh. Marinescu, 38, Tg-Mures, 540139
Z. Pávai
University of Medicine and Pharmacy
Email: rfechete@phys.utcluj.ro
Romênia, Gh. Marinescu, 38, Tg-Mures, 540139
D. Demco
Department of Physics and Chemistry, Technical University of Cluj-Napoca; DWI-Leibniz Institute for Interactive Materials, RWTH-Aachen University
Email: rfechete@phys.utcluj.ro
Romênia, 28 Memorandumului Street, Cluj-Napoca, 400114; e.V. Forckenbeckstraße 50, Aachen, 52056