The Effect of Hydroxynaphtazarin Derivatives on Decrease of Ischemic Area After Damage Focal Cerebral Blood Circulation

Abstract

7-Ethyl-2,3-diglutathionyl-6-hydroxynaphthazarin (GluNaz, 1a) can be a protector of the rat brain under ischemia. The effect of hydroxynaphtazarin derivative 1a was estimated. The focal ischemia by occlusion of middle cerebral artery (MCAo) was used. Conjugate 1a kept life of more then 30% of rats after treatment. Studied compounds did not render additional changes of arterial pressure before and within 3 h after operation. The injection of 1a in a concentration exceeding a therapeutic dose more than by ten times did not lead to an increase in heart rate in intact animals. The behavioral reactions of rats after occlusion showed an increase of cognitive frustration. Like 7-ethyl-2,3,6-trihydroxynaphthazarin (Ech, 1b) (Russian Pat. RU 2266737, 1999 [3]), GluNaz reduced the region of cytotoxic edema in rat brain after treatment. The recovering effect of 1a begins to improve within 48 h and 1b—within 72 h. Magnetic resonance imaging allows a detailed dynamic study of the effect of the compounds in our model of an ischemic stroke. So, the conjugate 1a studied can be potential medicine for the treatment of ischemic injury of a brain.