Isolation and Characterization of Thirty Polymorphic Microsatellite Markers from RAPD Product in Aspergillus niger and a Test of Cross-Species Amplification


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Thirty polymorphic simple sequence repeat (SSR) markers have been developed and characterized from RAPD product in Aspergillus niger in order to protect its natural resources. Polymorphisms of these RAPD-SSR markers were evaluated in a natural population of 25 strains collected from Shandong in China. The number of alleles (Na) per locus varied from 2 to 9. Observed (HO) and expected (HE) heterozygosities ranged from 0.56 to 0.96, and from 0.52 to 0.88, respectively. All the RAPD-SSR loci conformed to Hardy–Weinberg equilibrium after Bonferroni correction. They have showed sufficient level of polymorphisms to estimate the genetic diversity, population structure and species conservation in the A. niger. Five additional fungus species, Penicillium chrysogenum, Penicillium citrinum, Aspergillus flavus, Aspergillus ochratoxin and Aspergillus sulphureus were assessed for cross-species amplification. Three of the five species showed at least three polymorphic loci. In addition, eight loci were found to be polymorphic in at least one species.

Sobre autores

Yun-Guo Liu

College of Life Sciences, Linyi University; College of Life Sciences and Technology, Xinjiang University

Email: yguoliu@163.com
República Popular da China, Linyi, 276000; Urumqi, 830046

Qing-Dian Han

College of Life Sciences, Linyi University

Email: yguoliu@163.com
República Popular da China, Linyi, 276000

Yao-Yao Li

College of Life Sciences, Linyi University

Email: yguoliu@163.com
República Popular da China, Linyi, 276000

Ling-Xiao Liu

Linyi Academy of Agricultural Sciences

Autor responsável pela correspondência
Email: liulingxiao2002@163.com
República Popular da China, Linyi, 276012

Yu-You Li

Department of Civil and Environmental Engineering, Tohoku University

Autor responsável pela correspondência
Email: yguoliu@163.com
Japão, Sendai, 6-6-06

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