The molecular characteristic of multidrug-resistant strains of Mycobacterium tuberculosis isolated in Northwestern Russia

Аннотация

The goal of this work was to obtain genotypic characteristics of multidrug-resistant (MDR) strains of Mycobacterium tuberculosis (i.e., strains that are resistant at least to rifampicin and isoniazid) isolated from consumptives in Northwestern Russia in 2011–2012. Spoligotyping of 195 strains of Mycobacterium tuberculosis revealed 14 spolingotypes belonging to genetic families Beijing (n = 162), LAM (n = 15), H3/URAL (n = 14), T, Harleem, and X. Spolingotypes SIT1 (Beijing), SIT42 (LAM), and SIT62 (H3/URAL) were predominant. Regardless of genotype, all studied strains were resistant to streptomycin. Multidrug-resistant strains were resistant to ethionamide (56%), amikacin (31%), kanamycin (40%), and capreomycin (33%). The fractions of the strains resistant to ethambutol were 71 (n = 115) and 42% (n = 14) among the Beijing and nonBeijing strains, respectively (p < 0.05). Representatives of the Beijing genetic family remain predominant in Northwestern Russia in the population of multidrug-resistant strains of Mycobacterium tuberculosis (83%).