Species Diversity of Lactobacilli of Vaginal Microbiome: How to Observe It

Resumo

In this review, an attempt to trace the evolution of knowledge about the species diversity of lactobacilli in the historical perspective, from the moment of their discovery to the present, was made, taking into account the technical characteristics of experiments that can influence the final results of the research. Comparison of the data obtained using various methods of identification of lactobacilli was performed: after isolation based on preliminary cultivation using phenotypic and genotypic methods and based on direct identification of species by high-throughput sequencing and PCR methods.