Molecular genetic analysis of microorganisms with intraepithelial invasion isolated from patients with colorectal cancer


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Аннотация

The facultative aerobic bacteria isolated from the mucosa of rectum in the zone of malignant tumor and neighboring normal mucosa in patients with colorectal cancer were studied using molecular genetic methods. The species attribution of bacteria was implemented using the cultural morphological analysis and sequencing of the 16S rRNA locus. The microorganisms with intraepithelial invasion to rectal mucosa were identified as the representatives of adherent-invasive (AIEC) subgroup of Escherichia coli and species Klebsiella pneumonia. The molecular analysis by genetic determinants controlling adhesive, hemolytic, and toxigenic activity revealed that some bacterial isolates were able to produce toxins with potential cancerogenic activity (e.g., colibactin and cytotoxic necrotizing factor 1). Certain bacterial species isolated from malignant and normal rectal epithelium of the same patient demonstrated no difference between analyzed factors of toxigenicity.

Авторлар туралы

N. Nguyen

Kazan Federal University

Email: Olga.Ilinskaya@kpfu.ru
Ресей, Kazan, Tatarstan, 420008

R. Vafin

Kazan Federal University; Tatar Research Institute of Agriculture

Email: Olga.Ilinskaya@kpfu.ru
Ресей, Kazan, Tatarstan, 420008; Kazan, Tatarstan, 420059

I. Rzhanova

Kazan Federal University

Email: Olga.Ilinskaya@kpfu.ru
Ресей, Kazan, Tatarstan, 420008

A. Kolpakov

Kazan Federal University

Email: Olga.Ilinskaya@kpfu.ru
Ресей, Kazan, Tatarstan, 420008

I. Gataullin

Tatarstan Regional Clinical Cancer Center; Kazan State Medical Academy

Email: Olga.Ilinskaya@kpfu.ru
Ресей, Kazan, Tatarstan, 420002; Kazan, Tatarstan, 420012

S. Tyulkin

Tatar Interregional Veterinary Laboratory

Email: Olga.Ilinskaya@kpfu.ru
Ресей, Kazan, Tatarstan, 420087

M. Sinyagina

Kazan Federal University

Email: Olga.Ilinskaya@kpfu.ru
Ресей, Kazan, Tatarstan, 420008

T. Grigoryeva

Kazan Federal University

Email: Olga.Ilinskaya@kpfu.ru
Ресей, Kazan, Tatarstan, 420008

O. Ilinskaya

Kazan Federal University

Хат алмасуға жауапты Автор.
Email: Olga.Ilinskaya@kpfu.ru
Ресей, Kazan, Tatarstan, 420008

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© Allerton Press, Inc., 2016