Molecular genetic analysis of microorganisms with intraepithelial invasion isolated from patients with colorectal cancer
- Авторлар: Nguyen N.T.1, Vafin R.R.1,2, Rzhanova I.V.1, Kolpakov A.I.1, Gataullin I.G.3,4, Tyulkin S.V.5, Sinyagina M.N.1, Grigoryeva T.V.1, Ilinskaya O.N.1
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Мекемелер:
- Kazan Federal University
- Tatar Research Institute of Agriculture
- Tatarstan Regional Clinical Cancer Center
- Kazan State Medical Academy
- Tatar Interregional Veterinary Laboratory
- Шығарылым: Том 31, № 1 (2016)
- Беттер: 15-20
- Бөлім: Experimental Works
- URL: https://journals.rcsi.science/0891-4168/article/view/178020
- DOI: https://doi.org/10.3103/S0891416816010079
- ID: 178020
Дәйексөз келтіру
Аннотация
The facultative aerobic bacteria isolated from the mucosa of rectum in the zone of malignant tumor and neighboring normal mucosa in patients with colorectal cancer were studied using molecular genetic methods. The species attribution of bacteria was implemented using the cultural morphological analysis and sequencing of the 16S rRNA locus. The microorganisms with intraepithelial invasion to rectal mucosa were identified as the representatives of adherent-invasive (AIEC) subgroup of Escherichia coli and species Klebsiella pneumonia. The molecular analysis by genetic determinants controlling adhesive, hemolytic, and toxigenic activity revealed that some bacterial isolates were able to produce toxins with potential cancerogenic activity (e.g., colibactin and cytotoxic necrotizing factor 1). Certain bacterial species isolated from malignant and normal rectal epithelium of the same patient demonstrated no difference between analyzed factors of toxigenicity.
Авторлар туралы
N. Nguyen
Kazan Federal University
Email: Olga.Ilinskaya@kpfu.ru
Ресей, Kazan, Tatarstan, 420008
R. Vafin
Kazan Federal University; Tatar Research Institute of Agriculture
Email: Olga.Ilinskaya@kpfu.ru
Ресей, Kazan, Tatarstan, 420008; Kazan, Tatarstan, 420059
I. Rzhanova
Kazan Federal University
Email: Olga.Ilinskaya@kpfu.ru
Ресей, Kazan, Tatarstan, 420008
A. Kolpakov
Kazan Federal University
Email: Olga.Ilinskaya@kpfu.ru
Ресей, Kazan, Tatarstan, 420008
I. Gataullin
Tatarstan Regional Clinical Cancer Center; Kazan State Medical Academy
Email: Olga.Ilinskaya@kpfu.ru
Ресей, Kazan, Tatarstan, 420002; Kazan, Tatarstan, 420012
S. Tyulkin
Tatar Interregional Veterinary Laboratory
Email: Olga.Ilinskaya@kpfu.ru
Ресей, Kazan, Tatarstan, 420087
M. Sinyagina
Kazan Federal University
Email: Olga.Ilinskaya@kpfu.ru
Ресей, Kazan, Tatarstan, 420008
T. Grigoryeva
Kazan Federal University
Email: Olga.Ilinskaya@kpfu.ru
Ресей, Kazan, Tatarstan, 420008
O. Ilinskaya
Kazan Federal University
Хат алмасуға жауапты Автор.
Email: Olga.Ilinskaya@kpfu.ru
Ресей, Kazan, Tatarstan, 420008
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