Relationship of potential biomarkers of interstitial cystitis/painful bladder syndrome

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Abstract

BACKGROUND: Biomarker identification fulfills the need for simple, accurate, and non-invasive diagnostic methods for interstitial cystitis/painful bladder syndrome (IC/PBS). The simultaneous study of cytokines and growth factors is essential for understanding the role of biomarkers.

AIM: This work determines the concentration of interleukins (IL) 6 and 8, nerve growth factor (NGF), heparin-binding epidermal growth factor (HB-EGF), and their relationship in patients with IC/PBS.

MATERIALS AND METHODS: This study examined 126 female patients with IC/PBS, with a mean age of 46.7±14.0 years. The disease duration averaged 6.0±2.8 years. The control group consisted of 20 women without IC/PBS, with a mean age of 35.3±9.7 years. The level of IL-6 and IL-8 in blood serum and urine was determined by ELISA. The NGF and HB-EGF levels were determined by ELISA using the appropriate test kits. Statistical analysis was performed using SPSS software version 15.0. The Student’s t-test and Spearman’s correlation coefficient were calculated.

RESULTS: In patients with IC/PBS, blood levels of IL-6 and IL-8 exceeded the control indicator by 65.99% (t=4.62, p=0.000) and by 67.14% (t=2.81, p=0.006), respectively, the NGF level exceeded it by 8.82% (t=0.10, p=0.920), and the HB-EGF level decreased by 30.84% (t=0.62, p=0.537). In urine, the level of IL-6 in IC/PBS was increased by 52.94% (t=7.62, p=0.000), IL-8 level was increased by 57.53% (t=2.01, p=0.049), NGF was increased by 50.44% (t=2.06, p=0.043), and HB-EGF level was increased by 56.44% (t=2.01, p=0.049). Cytokines correlated with growth factors with weakly bound in blood and urine (p >0.05). A strong, direct relationship was registered between the urine levels of NGF and HB-EGF (r=+0.987, p <0.05).

CONCLUSIONS: Correlations between IL-6 and IL-8 with NGF and HB-EGF in urine indicate an increased risk of damage to the bladder epithelium. The increase in urinary HB-EGF is a response to a significant increase in urinary NGF and may be a bladder epithelial defense mechanism.

About the authors

Rashad F. Sholan

Republican Centre of Diagnosis and Treatment

Author for correspondence.
Email: ittihaf@yahoo.com
ORCID iD: 0000-0002-1047-167X

MD, Cand. Sci. (Med.)

Azerbaijan, Baku

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Supplementary files

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2. Fig. 1. The correlation between blood and urine levels of interleukin 8 (a) and blood and urine levels of interleukin 6 (b).

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3. Fig. 2. The correlation between blood and urine levels of nerve growth factor (NGF) (a) and blood and urine levels of heparin-binding epidermal growth factor (HB-EGF) (b).

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4. Fig. 3. The correlation between blood levels (a) and urinary levels (b) of nerve growth factor (NGF) and heparin-binding epidermal growth factor (HB-EGF).

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