The study of thymopoesis in children with thymomegalia using identification of thymus excisional rings


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Abstract

The article demonstrates that in children with thymomegalia the process of T-lymphopoesis in thymus is depressed. The functional condition of thymus was evaluated by detection of T-receptor excisional rings using polymerase chain reaction technique in real time. The T-receptor excisional rings are circular molecular structures developing in the process of rearrangement of V-genes of T-cell receptor. The most intensive and statistically reliable decreasing of content of T-receptor excisional rings in lymphocytes of blood is detected during first two years of life: 29.6 (18.1-41.0) copies per 1000 lymphocytes against 73.3 (54.0-83.8) copies in control. Hereinafter, (after two years) difference in level of T-receptor excisional rings inc children with thymomegalia in comparison with children without enlargement of thymus is less expressed i.e. it amounts to 1.8 times at the age before 10 years. In individuals of older age thymomegalia is a rather rare pathology. The examination of single patient aged of 40 years and with thymomegalia factually total absence of T-receptor excisional rings in cells of his blood was established.

About the authors

Almira D. Donetskova

The state research center institute of immunology of the Federal medical biological agency of Russia

Email: almira_donetskova@yahoo.com
MD, PhD, DSc 115478 Moscow, Russia

P. D Vaganov

The N.I. Pirogov Russian national research medical university Minzdrav of Russia

117997 Moscow, Russia

M. F Nikonova

The state research center institute of immunology of the Federal medical biological agency of Russia

115478 Moscow, Russia

E. Yu Yanovskaia

The N.I. Pirogov Russian national research medical university Minzdrav of Russia

117997 Moscow, Russia

E. T Mandzhieva

The N.I. Pirogov Russian national research medical university Minzdrav of Russia

117997 Moscow, Russia

E. M Rodionova

The N.I. Pirogov Russian national research medical university Minzdrav of Russia

117997 Moscow, Russia

I. M Donin

The Morosovskaia children municipal clinical hospital of the Moscow health department

119049 Moscow, Russia

M. A Pushkarev

The N.I. Pirogov Russian national research medical university Minzdrav of Russia

117997 Moscow, Russia

A. A Yarilin

The state research center institute of immunology of the Federal medical biological agency of Russia

115478 Moscow, Russia

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