A New Method for Express Detection of Antibiotic Resistance
- Авторлар: Pleskova S.1,2, Lazarenko E.1,2, Sudakova I.2, Kriukov R.1, Bezrukov N.1,2
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Мекемелер:
- N.I. Lobachevsky Nizhny Novgorod State University, Ministry of Science and Higher Education
- R.E. Alekseev Nizhny Novgorod State Technical University, Ministry of Science and Higher Education
- Шығарылым: Том 59, № 1 (2023)
- Беттер: 74-80
- Бөлім: Articles
- URL: https://journals.rcsi.science/0555-1099/article/view/138745
- DOI: https://doi.org/10.31857/S0555109923010075
- EDN: https://elibrary.ru/CVOIPK
- ID: 138745
Дәйексөз келтіру
Аннотация
The oscillation mode of an atomic force microscope (AFM) was used to create a highly sensitive real-time detection system for antibiotic resistance. This mode allows to evaluate the sensitivity or resistance of Gram-negative (Escherichia coli) and Gram-positive (Staphylococcus aureus) bacteria to an antibiotic in 15–30 minutes. The analytical signal (changes in the amplitude-frequency characteristics of the cantilever) is based on the metabolic activity of bacteria. Bacteria was adding on the cantilever and was causing it to oscillate with high amplitude. If the bacteria are sensitive to the antibiotic, the amplitude drops statistically significant within 15–30 minutes, if the bacteria are resistant, then the amplitude either does not change or increases. The obtained results were comparable with the disk diffusion method.
Авторлар туралы
S. Pleskova
N.I. Lobachevsky Nizhny Novgorod State University,Ministry of Science and Higher Education; R.E. Alekseev Nizhny Novgorod State Technical University,
Ministry of Science and Higher Education
Хат алмасуға жауапты Автор.
Email: pleskova@mail.ru
Russia, 603950, Nizhny Novgorod; Russia, 603115, Nizhny Novgorod
E. Lazarenko
N.I. Lobachevsky Nizhny Novgorod State University,Ministry of Science and Higher Education; R.E. Alekseev Nizhny Novgorod State Technical University,
Ministry of Science and Higher Education
Email: pleskova@mail.ru
Russia, 603950, Nizhny Novgorod; Russia, 603115, Nizhny Novgorod
I. Sudakova
R.E. Alekseev Nizhny Novgorod State Technical University,Ministry of Science and Higher Education
Email: pleskova@mail.ru
Russia, 603115, Nizhny Novgorod
R. Kriukov
N.I. Lobachevsky Nizhny Novgorod State University,Ministry of Science and Higher Education
Email: pleskova@mail.ru
Russia, 603950, Nizhny Novgorod
N. Bezrukov
N.I. Lobachevsky Nizhny Novgorod State University,Ministry of Science and Higher Education; R.E. Alekseev Nizhny Novgorod State Technical University,
Ministry of Science and Higher Education
Email: pleskova@mail.ru
Russia, 603950, Nizhny Novgorod; Russia, 603115, Nizhny Novgorod
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