A New Method for Express Detection of Antibiotic Resistance

S. N. Pleskova; Плескова С. Н.; E. V. Lazarenko; Лазаренко Е. В.; I. S. Sudakova; Судакова И. С.; R. N. Kriukov; Крюков Р. Н; N. A. Bezrukov; Безруков Н. А.

doi:10.31857/S0555109923010075

A New Method for Express Detection of Antibiotic Resistance

Authors: Pleskova S.N.¹^,2, Lazarenko E.V.¹^,2, Sudakova I.S.², Kriukov R.N.¹, Bezrukov N.A.¹^,2
Affiliations:
1. N.I. Lobachevsky Nizhny Novgorod State University, Ministry of Science and Higher Education
2. R.E. Alekseev Nizhny Novgorod State Technical University, Ministry of Science and Higher Education
Issue: Vol 59, No 1 (2023)
Pages: 74-80
Section: Articles
URL: https://journals.rcsi.science/0555-1099/article/view/138745
DOI: https://doi.org/10.31857/S0555109923010075
EDN: https://elibrary.ru/CVOIPK
ID: 138745

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Abstract

The oscillation mode of an atomic force microscope (AFM) was used to create a highly sensitive real-time detection system for antibiotic resistance. This mode allows to evaluate the sensitivity or resistance of Gram-negative (Escherichia coli) and Gram-positive (Staphylococcus aureus) bacteria to an antibiotic in 15–30 minutes. The analytical signal (changes in the amplitude-frequency characteristics of the cantilever) is based on the metabolic activity of bacteria. Bacteria was adding on the cantilever and was causing it to oscillate with high amplitude. If the bacteria are sensitive to the antibiotic, the amplitude drops statistically significant within 15–30 minutes, if the bacteria are resistant, then the amplitude either does not change or increases. The obtained results were comparable with the disk diffusion method.

Keywords

antibiotic resistance, atomic force microscopy, oscillation mode, disk diffusion method, Escherichia coli, Staphylococcus aureus

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