The Obtaining of Recombinant Producer of  Trametes hirsuta  Versatile Peroxidase VP2 in  Penicillium canescens

K. V. Moiseenko; Моисеенко К. В.; T. V. Fedorova; Федорова Т. В.; O. S. Savinova; Савинова О. С.; A. M. Chulkin; Чулкин А. М.

doi:10.31857/S0555109923060120

The Obtaining of Recombinant Producer of Trametes hirsuta Versatile Peroxidase VP2 in Penicillium canescens

Authors: Moiseenko K.V.¹, Fedorova T.V.¹, Savinova O.S.¹, Chulkin A.M.¹
Affiliations:
1. Bach Institute of Biochemistry, Biotechnology Research Center of Russian Academy of Sciences
Issue: Vol 59, No 6 (2023)
Pages: 605-613
Section: Articles
URL: https://journals.rcsi.science/0555-1099/article/view/232497
DOI: https://doi.org/10.31857/S0555109923060120
EDN: https://elibrary.ru/CJQZRR
ID: 232497

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Abstract
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Abstract

The interest in peroxidases of the basidiomycetes secreted enzyme complex is due to their wide substrate specificity and the ability of these enzymes to participate in the biodegradation of such difficultly degradable biopolymers as lignin. However, due to the difficulty of isolating these enzymes from native sources, their study is difficult. In this work, expression vectors were created that carried the sequence encoding the T. hirsuta LE-BIN072 versatile peroxidase VP2, which was transformed into the genome of the P. canescens strain. Screening of transformants showed the presence of peroxidase activity up to 1 U/mL. Fragments of the target protein in the culture liquid of the selected transformants were identified by mass spectrometric analysis. A new strain of P. canescens pVP2D-6, a producer of the recombinant universal peroxidase VP2 T. hirsuta LE-BIN072, was obtained for the first time, and the ability of the enzyme complex secreted by it to modify alkaline lignin was shown.

Keywords

peroxidase, lignolytic enzymes, Trametes hirsuta, Penicillium canescens, heterologous expression, lignin

References

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