Competitive Polarization Fluorescence Aptamer Analysis of Aflatoxin B1: Influence of Salt Composition of the Reaction Medium on the Interaction of Reagents and Detection Limit

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Abstract

The work examines the interaction of an aptamer (a synthetic receptor based on single-stranded DNA) having a loop structure with a specific ligand – aflatoxin B1 (AFB1), a common toxic contaminant of food products. The effects of mono- and divalent cations (Cs +, Li + , Na + , K +, Sr 2+, Ba 2+ , Mg 2+, Ca 2+) in different concentrations on the reactivity of the aptamer were studied. The fluorescence anisotropy (FA) of the fluorescein-labeled AFB1 derivative was recorded, reflecting both binding to the aptamer and the mobility of the aptamer – labeled AFB1 complex in a given reaction medium. It was shown that the recorded changes in FA differ significantly depending on the cation present in the reaction medium and its concentration. The effect on FA of monovalent cations was observed in the range of 400 mM–2.5 M, for divalent cations it is more pronounced and corresponds to concentrations from 6 to 200 mM. According to the degree of FA changes, the cations form the rows Cs + << K + ≤ ≤ Li + < Na + and Ba 2+ < Sr 2+ < Ca 2+ < Mg 2+. Comparison of the characteristics of the competitive determination of AFB1 for the traditional reaction medium and the medium with a 50-fold (from 20 mM to 1.0 M) increase in the concentration of magnesium acetate demonstrated that the given increase caused a 12-fold decrease in the detection limit – to 2.5 ± 0.4 nM. The results obtained allow considering choice of cation content as an effective tool for creating highly sensitive aptamer-based analytical systems.

About the authors

A. V. Samokhvalov

Bach Institute of Biochemistry, of Research Center of Biotechnology of the Russian Academy of Sciences

Moscow, 119071 Russia

A. V. Zherdev

Bach Institute of Biochemistry, of Research Center of Biotechnology of the Russian Academy of Sciences

Moscow, 119071 Russia

B. B. Dzantiev

Bach Institute of Biochemistry, of Research Center of Biotechnology of the Russian Academy of Sciences

Email: dzantiev@inbi.ras.ru
Moscow, 119071 Russia

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