DETECTION OF HEMOLYTIC STRAINS OF A EROMONADS BY MULTIPLEX PCR

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Abstract

This study presents a method for the co-amplification of α- and β-haemolysin genes in bacteria of the genus Aeromonas. The developed multiplex PCR protocol demonstrated high specificity and sensitivity. PCR screening showed a wide distribution of haemolysin genes among Aeromonas isolates isolated from aquaculture and environmental sites. A correlation was found between the haemolytic activity of strains and the presence of certain genes ( hlyA and aerA ). The proposed method may become a useful tool for epidemiological monitoring of the spread of pathogenic aeromonad species, which is important for ensuring biological safety of fish production.

About the authors

D. A. Dokolin

Demidov Yaroslavl State University, Laboratory of Ecobiomonitoring and Quality Control

Email: DimonDokolin@yandex.ru
Yaroslavl, 150003 Russia

M. N. Sokolov

Demidov Yaroslavl State University, Laboratory of Ecobiomonitoring and Quality Control

Email: DimonDokolin@yandex.ru
Yaroslavl, 150003 Russia

A. A. Payuta

Demidov Yaroslavl State University, Laboratory of Ecobiomonitoring and Quality Control

Email: DimonDokolin@yandex.ru
Yaroslavl, 150003 Russia

E. A. Fleurova

Demidov Yaroslavl State University, Laboratory of Ecobiomonitoring and Quality Control

Email: DimonDokolin@yandex.ru
Yaroslavl, 150003 Russia

Y. V. Zaitseva

Demidov Yaroslavl State University, Laboratory of Ecobiomonitoring and Quality Control

Author for correspondence.
Email: DimonDokolin@yandex.ru
Yaroslavl, 150003 Russia

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