Formation of Various Antimicrobial Peptide Emericellipsin Isoforms in  Emericellopsis alkalina  under Different Cultivation Conditions

A. E. Kuvarina; Куварина А. Е.; M. A. Sukonnikov; Суконников М. А.; E. A. Rogozhin; Рогожин Е. А.; M. V. Serebryakova; Серебрякова М. В.; A. V. Timofeeva; Тимофеева А. В.; M. L. Georgieva; Георгиева М. Л.; V. S. Sadykova; Садыкова В. С.

doi:10.31857/S0555109923020095

Formation of Various Antimicrobial Peptide Emericellipsin Isoforms in Emericellopsis alkalina under Different Cultivation Conditions

Authors: Kuvarina A.E.¹, Sukonnikov M.A.¹, Rogozhin E.A.¹^,2, Serebryakova M.V.³, Timofeeva .V.³, Georgieva M.L.¹^,4, Sadykova V.S.¹
Affiliations:
1. Gause Institute New Antibiotics
2. Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry
3. Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University
4. Lomonosov Moscow State University
Issue: Vol 59, No 2 (2023)
Pages: 174-181
Section: Articles
URL: https://journals.rcsi.science/0555-1099/article/view/138756
DOI: https://doi.org/10.31857/S0555109923020095
EDN: https://elibrary.ru/LLSKOD
ID: 138756

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Abstract

A microbiological screening of the target component of emericellipsin A of the Emericellopsis alkalina E101 strain was carried out in various biotechnological systems at various pH. The content of emericellipsin A was quantified under these conditions.It has been established that the new approved membrane-liquid cultivation method at pH 10 contributes to an increase in the yield of the main component of emericellipsin A. It was shown that the new method of cultivating the strain E. alkalina E101 also promotes the synthesis of various isoforms of the main component of emericellipsin A. Some comparative analysis of them was carried out.

Keywords

antibiotics, Emericellopsis alkalina, emericellipsin A, isoforms of emericellipsin A

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