Formation of Various Antimicrobial Peptide Emericellipsin Isoforms in Emericellopsis alkalina under Different Cultivation Conditions
- Authors: Kuvarina A.E.1, Sukonnikov M.A.1, Rogozhin E.A.1,2, Serebryakova M.V.3, Timofeeva .V.3, Georgieva M.L.1,4, Sadykova V.S.1
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Affiliations:
- Gause Institute New Antibiotics
- Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry
- Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University
- Lomonosov Moscow State University
- Issue: Vol 59, No 2 (2023)
- Pages: 174-181
- Section: Articles
- URL: https://journals.rcsi.science/0555-1099/article/view/138756
- DOI: https://doi.org/10.31857/S0555109923020095
- EDN: https://elibrary.ru/LLSKOD
- ID: 138756
Cite item
Abstract
A microbiological screening of the target component of emericellipsin A of the Emericellopsis alkalina E101 strain was carried out in various biotechnological systems at various pH. The content of emericellipsin A was quantified under these conditions.It has been established that the new approved membrane-liquid cultivation method at pH 10 contributes to an increase in the yield of the main component of emericellipsin A. It was shown that the new method of cultivating the strain E. alkalina E101 also promotes the synthesis of various isoforms of the main component of emericellipsin A. Some comparative analysis of them was carried out.
About the authors
A. E. Kuvarina
Gause Institute New Antibiotics
Author for correspondence.
Email: nastena.lysenko@mail.ru
Russia, 119021, Moscow
M. A. Sukonnikov
Gause Institute New Antibiotics
Email: sadykova_09@mail.ru
Russia, 119021, Moscow
E. A. Rogozhin
Gause Institute New Antibiotics; Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry
Email: sadykova_09@mail.ru
Russia, 119021, Moscow; Russia, 117997, Moscow
M. V. Serebryakova
Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University
Email: sadykova_09@mail.ru
Russia, 119234, Moscow
A. V. Timofeeva
Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University
Email: sadykova_09@mail.ru
Russia, 119234, Moscow
M. L. Georgieva
Gause Institute New Antibiotics; Lomonosov Moscow State University
Email: sadykova_09@mail.ru
Russia, 119021, Moscow; Russia, 119234, Moscow
V. S. Sadykova
Gause Institute New Antibiotics
Author for correspondence.
Email: sadykova_09@mail.ru
Russia, 119021, Moscow
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