Development of Fluorescence Polarization Immunoassay of Sulfathiazol and its Application for Honey Testing

I. S. Nesterenko; Нестеренко И. С.; O. D. Hendrickson; Гендриксон О. Д.; N. I. Smirnova; Смирнова Н. И.; S. A. Eremin; Еремин С. А.; D. V. Sotnikov; Сотников Д. В.

doi:10.31857/S0555109923010051

Development of Fluorescence Polarization Immunoassay of Sulfathiazol and its Application for Honey Testing

Authors: Nesterenko I.S.¹, Hendrickson O.D.², Smirnova N.I.², Eremin S.A.², Sotnikov D.V.²
Affiliations:
1. The Russian State Center for Animal Feed and Drug Standardization and Quality
2. Bach Institute of Biochemistry, Research Center of Biotechnology of the Russian Academy of Sciences
Issue: Vol 59, No 1 (2023)
Pages: 96-102
Section: Articles
URL: https://journals.rcsi.science/0555-1099/article/view/138749
DOI: https://doi.org/10.31857/S0555109923010051
EDN: https://elibrary.ru/CUSONF
ID: 138749

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Abstract
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Abstract

A fluorescence polarization immunoassay has been developed for the detection of the antibacterial sulfathiazole. The latter belongs to the sulfonamide class of antibacterial, which is used in beekeeping for the prevention and treatment of infectious diseases of bees. The assay conditions, which ensure the achievement of high analytical characteristics, are determined. The duration of the analysis is 5 min; the limit of sulfathiazole detection is 3 ng/mL. A study of the assay selectivity concerning other sulfonamides demonstrated the high specificity only towards sulfathiazole. The optimal method of sample preparation before the control honey contamination was proposed. The effectiveness of the developed analysis for the detection of sulfathiazole in honey samples was confirmed. The developed fluorescent polarization immunoassay is a rapid and efficient analytical system for rapid and sensitive control of honey quality and safety.

Keywords

sulfathiazole, antibiotics, fluorescence polarization immunoassay, food quality control, honey

References

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