New Inhibitors of Pancreatic α-amylase from  Rhaponticum uniflorum

D. N. Olennikov; Оленников Д. Н.; N. I. Kashchenko; Кащенко Н. И.

doi:10.31857/S0555109923010063

New Inhibitors of Pancreatic α-amylase from Rhaponticum uniflorum

Authors: Olennikov D.N.¹, Kashchenko N.I.¹
Affiliations:
1. Institute of General and Experimental Biology SD RAS
Issue: Vol 59, No 1 (2023)
Pages: 46-55
Section: Articles
URL: https://journals.rcsi.science/0555-1099/article/view/138740
DOI: https://doi.org/10.31857/S0555109923010063
EDN: https://elibrary.ru/CUYJJV
ID: 138740

Cite item

Full Text

Open Access
Restricted Access

Access granted
Restricted Access

Subscription Access

Abstract
About the authors
References
Supplementary files
Statistics

Abstract

The basic strategy for the treatment of diabetes mellitus is the control of postprandial glucose levels, and therefore pancreatic α-amylase that hydrolyzes complex carbohydrates is an important enzymatic target for the scientific research. In the present study, an aqueous extract and its ethyl acetate fraction (EAF) from the seeds of Rhaponticum uniflorum had a pronounced inhibitory effect on the activity of human pancreatic α-amylase. Sixteen metabolites were isolated after the chromatographic separation of EAF and characterized as 4-O-, 5-O-, 3,4-di-O-, 3,5-di-O-, 4,5-di-O-caffeoylquinic acid, 6-hydroxyluteolin 7-O-glucoside, rhaunoside B, luteolin 7-O-(6"-O-caffeoyl)-glucoside, luteolin, carthamoside, carthamogenin, tracheloside, isoferuloyl-serotonin, 20-hydroxyecdysone, 2-deoxy-20-hydroxyecdysone and a new natural compound, which was carthamogenin 4-O-(6''-O-acetyl)-β-D-glucopyranoside (6''-O-acetyl-cartamoside). Quantitative HPLC data indicated a different distribution of the individual components between the endosperm and the seed coat. The study of the compounds effect on the activity of human pancreatic α-amylase showed that some flavonoids, caffeoylquinic acids, lignans and serotonin derivatives had a pronounced inhibitory effect. These results support the conclusion that Rhaponticum uniflorum seeds may be a useful natural source for the development of α-amylase inhibitory agents.

Keywords

Rhaponticum uniflorum, human pancreatic α-amylase inhibitors, phenolic compounds

About the authors

D. N. Olennikov

Institute of General and Experimental Biology SD RAS

Author for correspondence.
Email: olennikovdn@mail.ru
Russia, 670047, Ulan-Ude

N. I. Kashchenko

Institute of General and Experimental Biology SD RAS

Email: olennikovdn@mail.ru
Russia, 670047, Ulan-Ude

References

Alam S., Sarker M.M.R., Sultana T.N., Chowdhury M.N.R., Rashid M.A., Chaity N.I. et al. // Front. Endocrinol. 2022. V. 13. № 800714. https://doi.org/10.3389/fendo.2022.800714
Sales P.M., Souza P.M., Simeoni L.A., Magalhães P.O., Silveira D. // J. Pharm. Pharm. Sci. 2009. V. 15. P. 141–183. https://doi.org/10.18433/j35s3k
Баторова С.М., Яковлев Г.П., Асеева Т.А. Справочник лекарственных растений традиционной тибетской медицины. Новосибирск: Наука, 2003. 291 с.
Olennikov D.N., Kashchenko N.I. // Chem. Nat. Comp. 2019. V. 55. P. 256–264. https://doi.org/10.1007/s10600-019-02662-2
Olennikov D.N. // Chem. Nat. Comp. 2018. V. 54. P. 751–754. https://doi.org/10.1007/s10600-018-2462-4
Olennikov D.N. // Chem. Nat. Comp. 2019. V. 55. P. 157–159. https://doi.org/10.1007/s10600-019-02642-6
Shantanova L.N., Olennikov D.N., Matkhanov I.E., Gulyaev S.M., Toropova A.A., Nikolaeva I.G., Nikolaev S.M. // Pharmaceuticals. 2021. V. 14. № 1186. https://doi.org/10.3390/ph14111186
Olennikov D.N., Chemposov V.V., Chirikova N.K. // Plants. 2021. V. 10. № 2525. https://doi.org/10.3390/plants10112525
Harmatha J., Buděšínský M., Vokáč K., Pavlik M., Grüner K., Laudová V. // Collect. Czech. Chem. Commun. 2007. V. 72. P. 334–346. https://doi.org/10.1135/cccc20070334
Olennikov D.N., Chirikova N.K., Kashchenko N.I., Gornostai T.G., Selyutina I.Y., Zilfikarov I.N. // Int. J. Mol. Sci. 2017. V. 18. № 2579. https://doi.org/10.3390/ijms18122579
Akabane M., Yamamoto A., Aizawa S., Taga A., Kodama S. // Analyt. Sci. 2014. V. 30. P. 739–743. https://doi.org/10.2116/analsci.30.739
Olennikov D.N., Chirikova N.K. // Chem. Nat. Comp. 2019. V. 55. P. 1032–1038. https://doi.org/10.1007/s10600-019-02887-1
Olennikov D.N., Chirikova N.K., Kashchenko N.I., Nikolaev V.M., Kim S.-W., Vennos C. // Front. Pharmacol. 2018. V. 9. № 756. https://doi.org/10.3389/fphar.2018.00756
Sólyomváry A., Mervai Z., Molnár-Perl I., Boldizsár I. // Nat. Prod. Res. 2014. V. 28. P. 732–739. https://doi.org/10.1080/14786419.2013.879473
Tadera K., Minami Y., Takamatsu K., Matsuoka T. // J. Nutr. Sci. Vitaminol. 2006. V. 52. P. 149–153. https://doi.org/10.3177/jnsv.52.149
Dandekar P.D., Kotmale A.S., Chavan S.R., Kadlag P.P., Sawant S.V., Dhavale D.D., Kumar A.R. // ACS Omega. 2021. V. 6. P. 1780–1786. https://doi.org/10.1021/acsomega.0c00617
Ponnusamy S., Zinjarde S., Bhargava S., Rajamohanan P.R., Ravikumar A. // Food Chem. 2012. V. 135. P. 2638–2642. https://doi.org/10.1016/j.foodchem.2012.06.110
García A.L.L., Olaya M.Q.J.H., Sierra A.J.I. // Rev. Cubana Plant. Med. 2017. V. 22. P. 1–14.
Cansian R.L., Vanin A.B., Orlando T., Piazza S.P., Puton B.M.S., Cardoso R.I. et al. // Braz. J. Biol. 2017. V. 77. P. 155–161. https://doi.org/10.1590/1519-6984.12215
Yamasaki T., Sato M., Mori T., Mohamed A.S.A., Fujii K., Tsukioka J. // J. Nat. Toxins. 2002. V. 11. P. 165–171.