Enzyme immunoassay system for serological diagnosis of hemorrhagic fever with renal syndrome based on inactivated purified Puumala virus (Hantaviridae: Orthohantavirus)
- 作者: Ivanov A.P.1, Dzagurova T.K.1, Kurashova S.S.1, Teodorovich R.D.1, Klebleeva T.D.1, Tkachenko E.A.1
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隶属关系:
- Chumakov Federal Scientific Center for Research and Development of Immune-and-Biological Products of the Russian Academy of Sciences (Institute of Poliomyelitis)
- 期: 卷 69, 编号 3 (2024)
- 页面: 285-289
- 栏目: TO VIROLOGIST’S AID
- URL: https://journals.rcsi.science/0507-4088/article/view/259220
- DOI: https://doi.org/10.36233/0507-4088-230
- EDN: https://elibrary.ru/mgtslj
- ID: 259220
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Introduction. Hemorrhagic fever with renal syndrome (HFRS) is the most common zoonotic human viral disease in the Russian Federation. More than 98% of the HFRS cases are caused by Puumala orthohantavirus (PUU). Effective serological tests are required for laboratory diagnosis of HFRS.
Objective. Construction of an enzyme immunoassay (ELISA) test system for detection of specific antibodies using standard antigen in the form of highly purified inactivated PUU virus as immunosorbent.
Materials and methods. Preparation of PUU virus antigen, designing the ELISA for detection of specific antibodies, developing parameters of the ELISA system, parallel titration of HFRS patients sera by fluorescent antibody technique (FAT) and the new ELISA.
Results and discussion. For the first time, ELISA based on purified inactivated PUU virus as standard antigen directly absorbed onto immunoplate was developed. Parallel titration of 50 samples from HFRS patients blood sera using FAT and the developed ELISA showed high sensitivity and specificity of this ELISA, with 100% concordance of testing results and significant level of correlation between the titers of specific antibodies in the two assays.
Conclusion. The ELISA based on purified inactivated PUU virus as an immunosorbent can be effectively used for HFRS serological diagnosis and for mass seroepidemiological studies.
作者简介
Alexander Ivanov
Chumakov Federal Scientific Center for Research and Development of Immune-and-Biological Products of the Russian Academy of Sciences (Institute of Poliomyelitis)
编辑信件的主要联系方式.
Email: ivanovalexander1@gmail.com
ORCID iD: 0000-0003-4576-7136
D. Sc. (Medicine), Leading Researcher, Laboratory of Molecular Biology of Viruses
俄罗斯联邦, MoscowTamara Dzagurova
Chumakov Federal Scientific Center for Research and Development of Immune-and-Biological Products of the Russian Academy of Sciences (Institute of Poliomyelitis)
Email: dzaguron@gmail.com
ORCID iD: 0000-0002-6656-1682
D. Sc. (Medicine), Leading Researcher, Laboratory of Hemorrhagic Fevers
俄罗斯联邦, MoscowSvetlana Kurashova
Chumakov Federal Scientific Center for Research and Development of Immune-and-Biological Products of the Russian Academy of Sciences (Institute of Poliomyelitis)
Email: svetllanak@yandex.ru
ORCID iD: 0000-0001-9934-699X
Cand. Sc. (Medicine), Leading Researcher, Laboratory of Hemorrhagic Fevers
俄罗斯联邦, MoscowRostislav Teodorovich
Chumakov Federal Scientific Center for Research and Development of Immune-and-Biological Products of the Russian Academy of Sciences (Institute of Poliomyelitis)
Email: rostislavteo@mail.ru
ORCID iD: 0000-0003-2117-597X
Researcher, Laboratory of Hemorrhagic Fevers
俄罗斯联邦, MoscowTatyana Klebleeva
Chumakov Federal Scientific Center for Research and Development of Immune-and-Biological Products of the Russian Academy of Sciences (Institute of Poliomyelitis)
Email: klebleeva_td@chumakovs.su
ORCID iD: 0000-0003-4288-6818
Researcher, Laboratory of Molecular Biology of Viruses
俄罗斯联邦, MoscowEugeny Tkachenko
Chumakov Federal Scientific Center for Research and Development of Immune-and-Biological Products of the Russian Academy of Sciences (Institute of Poliomyelitis)
Email: evgeniytkach@mail.ru
ORCID iD: 0000-0002-6829-1241
D. Sc. (Med), Professor, Head of the Scientific Direction
俄罗斯联邦, Moscow参考
- Dzagurova T.K., Tkachenko E.A., Petrov V.A. The effectiveness of the use of cultural antigens for serodiagnostics of GLPS using the immunofluorescence method. Voprosy virusologii. 1988; 33(1): 71–5. EDN: https://elibrary.ru/jfmqvh (in Russian)
- Ivanov A.P., Tkachenko E.A., Petrov V.A., Pashkov A.J., Dzagurova T.K., Vladimirova T.P., et al. Enzyme immunoassay for the detection of virus specific Ig G and Ig M antibody in patients with hemorrhagic fever with renal syndrome. Arch. Virol. 1988; 100: 1–7. DOI: https://doi.org/10.1007/BF01310902
- Rezapkin G.V., Tkachenko E.A., Ivanov A.P., Bashkirtsev V.N., Dzagurova T.K. Determination of arenavirus antigens and antibodies by solid-phase radioimmunological analysis. Voprosy virusologii. 1981; 26(4): 459–63. (in Russian)
- Tkachenko E.A., Ishmukhametov A.A., Dzagurova T.K., Sinyugina A.A., Korotina N.A., Nabatnikov P.A., et al. Manufacturing techniques and methods of control of the inactivated Vero cell-derived vaccine against HFRS has been developed in Russia. Remedium. 2015; (6): 47–54. EDN: https://elibrary.ru/uabrmj (in Russian)
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