Bastroviruses (Astroviridae): genetic diversity and potential impact on human and animal health

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Introduction. Bastroviruses were discovered in the Netherlands in 2016 in human stool samples and show partial genetic similarities to astroviruses and hepatitis E viruses. Their association with disease onset has not yet been established.

Materials and methods. Metagenomic sequencing of fecal samples of Nyctalus noctula bats collected in the Russian Federation in 2023 was performed. Two almost complete genomes of bastroviruses were assembled. The zoonotic potential of these viruses was assessed using machine learning methods, their recombination was studied, and phylogenetic trees were constructed.

Results. A nearly complete bastrovirus genome was de novo assembled in one of the samples, and it was used to assemble another genome in another sample. The zoonotic potential of the virus from one of these samples was estimated as high. The existence of recombination between structural and non-structural polyproteins was demonstrated.

Conclusion. Two bastrovirus genomes were assembled, phylogenetic and recombination analyses were performed, and the zoonotic potential was evaluated.

作者简介

German Roev

Central Research Institute for Epidemiology of the Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing; Moscow Institute of Physics and Technology (National Research University)

Email: roevherman@gmail.com
ORCID iD: 0000-0002-2353-5222

Bioinformatician of Laboratory for Genomics Research of the Central Research Institute for Epidemiology of the Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing, Moscow, Russia

俄罗斯联邦, 111123, Moscow; 115184, Dolgoprudny

Nadezhda Borisova

Central Research Institute for Epidemiology of the Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing

Email: borisova@cmd.su
ORCID iD: 0000-0002-9672-0648

Junior researcher of Laboratory for Genomics Research of the Central Research Institute for Epidemiology of the Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing, Moscow, Russia

俄罗斯联邦, 111123, Moscow

Nadezhda Chistyakova

A.N. Severtsov Institute of Ecology and Evolution of the Russian Academy of Sciences

Email: lanche@mail.ru
ORCID iD: 0009-0002-6034-1408

Engineer of Laboratory of comparative etology and biocommunication of A.N. Severtsov Institute of Ecology and Evolution of the Russian Academy of Sciences, Russia, Moscow, Russia

俄罗斯联邦, 119071, Moscow

Anastasia Vyhodtseva

Central Research Institute for Epidemiology of the Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing

Email: vihodceva@cmd.su
ORCID iD: 0009-0005-1911-9620

Technologist of Laboratory for Genomics Research of the Central Research Institute for Epidemiology of the Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing, Moscow, Russia

俄罗斯联邦, 111123, Moscow

Vasiliy Akimkin

Central Research Institute for Epidemiology of the Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing

Email: vgakimkin@yandex.ru
ORCID iD: 0000-0003-4228-9044

Doctor of Medicine, Professor, Director of Central Research Institute for Epidemiology of the Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing, Moscow, Russia

俄罗斯联邦, 111123, Moscow

Kamil Khafizov

Central Research Institute for Epidemiology of the Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing

编辑信件的主要联系方式.
Email: khafizov@cmd.su
ORCID iD: 0000-0001-5524-0296

PhD (Biol.), Head of Laboratory for Genomics Research of the Central Research Institute for Epidemiology of the Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing, Moscow, Russia

俄罗斯联邦, 111123, Moscow

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2. Fig. 1. Phylogenetic tree of the non-structural polyprotein (NSP) of bastroviruses.

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3. Fig. 2. Phylogenetic tree of the structural polyprotein (SP) of bastroviruses.

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4. Fig. 3. Phylogenetic compatibility matrix constructed from the merged alignments of NSP and SP proteins.

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版权所有 © Roev G.V., Borisova N.I., Chistyakova N.V., Vyhodtseva A.V., Akimkin V.G., Khafizov K.F., 2023

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