Antiviral activity of extracts of basidiomycetes and humic compounds substances against Human Immunodeficiency Virus (Retroviridae: Orthoretrovirinae: Lentivirus: Human immunodeficiency virus 1) and Herpes Simplex Virus (Herpesviridae: Simplexvirus: Human alphaherpesvirus 1)
- 作者: Nosik D.1, Nosik N.1, Teplyakova T.2, Lobach O.1, Kiseleva I.1, Kondrashina N.1, Bochkova M.1, Ananko G.2
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隶属关系:
- The D.I. Ivanovsky Institute of Virology FSBI «National Research Center of Epidemiology and Microbiology named after honorary academician N.F. Gamaleya» of the Ministry of Health of Russia
- FBU State Research Center of Virology and Biotechnology «Vector» of Rospotrebnadzor
- 期: 卷 65, 编号 5 (2020)
- 页面: 276-283
- 栏目: ORIGINAL RESEARCH
- URL: https://journals.rcsi.science/0507-4088/article/view/118142
- DOI: https://doi.org/10.36233/0507-4088-2020-65-5-4
- ID: 118142
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Introduction. One of the most urgent problem of modern medicine is the fight against the disease caused by the Human Immunodeficiency Virus (HIV) – HIV infection. The chemical compounds have improved the situation for infected people, but they are toxic, disrupt the metabolism and cannot eliminate the integrated virus from the body. The emergence of resistant HIV strains makes these treatments ineffective. Often, the death of HIV-infected people occurs as a result of the development of opportunistic infections caused by viruses of the Herpesviridae family. Therefore, the search for new therapeutic and preventive drugs that are less toxic and active against several viruses at the same time is relevant. Basidiomycetes, higher fungi, are a source of medicinal compounds that have antimicrobial properties, as well as antiviral ones. Humic compounds (HS) of various nature also have antiviral activity.
The aim of the study was to obtain nontoxic compounds from the basidiomycete Inonotus obliquus and humic compounds from brown coals and to test their activity against viruses that are pathogenic to humans: HIV and Herpes Simplex Virus (HSV).
Material and methods. The antiviral activity of melanin extracts obtained from the culture of the chaga fungus Inonotus obliquus and HS from the brown coal of the Kansko-Achinsk Deposit was studied using a model of MT-4 lymphoblastoid cells infected with HIV type 1 (HIV–1) strains and a monolayer culture of Vero cells infected with HSV type 1 (HSV-1) using virological and statistical research methods.
Results and discussion. It was found that all the studied compounds did not have a cytotoxic effect on cells at a concentration of 100 mcg/ml. It was shown that extracts of basidiomycetes and HS have antiviral activity against HIV-1 and HSV-1. EC 50 (50%-effective concentration) for HIV-1 was 3.7–5.0 mcg/ml, selectivity index 28–35. Antiherpetic activity was detected at a dose of 50–100 mcg/ml. The antiviral effectiveness of melanin compounds was established both in the «preventive» (2 hours before cell infection) and in the «therapeutic» regimen of drug administration, both for HIV-1 and HSV-1. The presence of antiviral activity of melanin and HS in relation to the RNA-containing HIV-1 virus and DNA-containing HSV-1 virus in our study coincides with the results of a number of authors in relation to influenza viruses, herpes virus, HIV, hepatitis B virus, Coxsackievirus, smallpox vaccine virus, which suggests that the type of nucleic acid in the virus does not play a fundamental role in the antiviral action of these drugs. It is also clear that HS is effective against both enveloped and non-enveloped viruses.
Conclusion. In general, it can be concluded that melanin and humic compounds are characterized by low toxicity in the presence of both virucidal and antiviral activity. This allows us to consider the studied compounds as the basis for creating safe medicines that are effective against pathogens of various viral infections.
作者简介
D. Nosik
The D.I. Ivanovsky Institute of Virology FSBI «National Research Center of Epidemiology and Microbiology named after honorary academician N.F. Gamaleya» of the Ministry of Health of Russia
Email: dnnosik@yandex.ru
ORCID iD: 0000-0001-5757-5671
123098, Moscow 俄罗斯联邦
N. Nosik
The D.I. Ivanovsky Institute of Virology FSBI «National Research Center of Epidemiology and Microbiology named after honorary academician N.F. Gamaleya» of the Ministry of Health of Russia
Email: nosiknn@yandex.ru
ORCID iD: 0000-0003-1943-6536
123098, Moscow 俄罗斯联邦
T. Teplyakova
FBU State Research Center of Virology and Biotechnology «Vector» of Rospotrebnadzor
Email: vector@vector.nsc.ru
ORCID iD: 0000-0003-4754-5051
630559, Koltsovo district, Novosibirsk region 俄罗斯联邦
O. Lobach
The D.I. Ivanovsky Institute of Virology FSBI «National Research Center of Epidemiology and Microbiology named after honorary academician N.F. Gamaleya» of the Ministry of Health of Russia
编辑信件的主要联系方式.
Email: victoriola@yandex.ru
ORCID iD: 0000-0001-9351-6433
Cand. Sci. Biol., senior researcher at the Laboratory of antiviral and disinfection agents
123098, Moscow
俄罗斯联邦I. Kiseleva
The D.I. Ivanovsky Institute of Virology FSBI «National Research Center of Epidemiology and Microbiology named after honorary academician N.F. Gamaleya» of the Ministry of Health of Russia
Email: iakiseleva@yandex.ru
ORCID iD: 0000-0003-3693-6081
123098, Moscow 俄罗斯联邦
N. Kondrashina
The D.I. Ivanovsky Institute of Virology FSBI «National Research Center of Epidemiology and Microbiology named after honorary academician N.F. Gamaleya» of the Ministry of Health of Russia
Email: nina.kond1950@yandex.ru
ORCID iD: 0000-0003-3985-3839
123098, Moscow 俄罗斯联邦
M. Bochkova
The D.I. Ivanovsky Institute of Virology FSBI «National Research Center of Epidemiology and Microbiology named after honorary academician N.F. Gamaleya» of the Ministry of Health of Russia
Email: bochkovams1@yandex.ru
ORCID iD: 0000-0001-9295-8379
123098, Moscow 俄罗斯联邦
G. Ananko
FBU State Research Center of Virology and Biotechnology «Vector» of Rospotrebnadzor
Email: vector@vector.nsc.ru
ORCID iD: 0000-0001-6570-5501
630559, Koltsovo district, Novosibirsk region 俄罗斯联邦
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