Modeling influenza virus infection in mature Wistar rats
- Authors: Marchenko V.A.1, Barashkova S.V.2, Zelinskaya I.A.2, Toropova Y.G.2, Sorokin E.V.1, Zhilinskaya I.N.1
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Affiliations:
- Smorodintsev Research Institute of Influenza
- Almazov National Medical Research Centre
- Issue: Vol 65, No 3 (2020)
- Pages: 159-166
- Section: ORIGINAL RESEARCH
- URL: https://journals.rcsi.science/0507-4088/article/view/118141
- DOI: https://doi.org/10.36233/0507-4088-2020-65-3-159-166
- ID: 118141
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Abstract
It has now been established that blood vessels are target for influenza, but the mechanism by which the influenza virus affects the cardiovascular system is unknown.
The aim – adaptation of influenza virus A/St. Petersburg/48/16 H1N1(pdm09) to mature Wistar rats, as these animals are the main experimental model for studying the pathology of the cardiovascular system.
Material and methods. Passage of influenza A virus (IAV) in embryonated chicken eggs, intranasal inoculation of rats with virus-containing material s, production of pulmonary homogenate, determination of IAV titer in embryonated chicken eggs, detection of histological changes in lung and pulmonary vessels.
Results. The article presents the results of the adaptation of influenza virus A/St. Petersburg/48/16 H1N1(pdm09) to mature Wistar rats. The infectious titer of the virus in the homogenates of infected rats lungs at the last stage of adaptation was 7.0 lg EID50/ml. Histological studies revealed pronounced changes in the respiratory tract (spasm of bronchioles, submucosal edema, desquamation of ciliated epithelium of bronchioles) and pulmonary vessels (spasm, desquamation and swelling of endotheliocytes, dissociation and swelling of the elastic membrane and media). In order to identify IAV in blood vessels and lung tissues, an immunohistochemical study was performed using monoclonal antibodies to NP antigen of IAV.
Conclusion. The data obtained allow us to conclude that the strain of influenza virus A/St. Petersburg/48/16 H1N1(pdm09) was adapted to mature Wistar rats maintaining virulent properties. The infectious titer of the virus at the last stage of adaptation was 7.0 lg EID50/ml. IAV identification is confirmed by immunohistochemical examination.
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##article.viewOnOriginalSite##About the authors
V. A. Marchenko
Smorodintsev Research Institute of Influenza
Author for correspondence.
Email: vmarcenco@mail.ru
ORCID iD: 0000-0001-6870-3157
Vladimir A. Marchenko, graduate student, research assistant of Laboratory of systemic virology
Saint Petersburg, 197376
Russian FederationS. V. Barashkova
Almazov National Medical Research Centre
Email: fake@neicon.ru
ORCID iD: 0000-0002-5618-4510
Svetlana V. Barashkova
Saint Petersburg, 197341
Russian FederationI. A. Zelinskaya
Almazov National Medical Research Centre
Email: fake@neicon.ru
ORCID iD: 0000-0002-1971-3444
Irina A. Zelinskaya
Saint Petersburg, 197341
Russian FederationY. G. Toropova
Almazov National Medical Research Centre
Email: fake@neicon.ru
ORCID iD: 0000-0003-1629-7868
Yana G. Toropova
Saint Petersburg, 197341
Russian FederationE. V. Sorokin
Smorodintsev Research Institute of Influenza
Email: fake@neicon.ru
ORCID iD: 0000-0003-1732-1727
Evgeniy V. Sorokin
Saint Petersburg, 197376
Russian FederationI. N. Zhilinskaya
Smorodintsev Research Institute of Influenza
Email: fake@neicon.ru
ORCID iD: 0000-0002-0084-1323
Irina N. Zhilinskaya
Saint Petersburg, 197376
Russian FederationReferences
- Opie E.L. The pathologic anatomy of influenza based chiefly on American and British sources. Arch. Pathol. Lab. Med. 1928; 5(2): 285-303.
- Lobo S.M., Watanabe A.S.A., Salomão M.L.M., Queiroz F., Gandolfi J.V., de Oliveira N.E., et al. Excess mortality is associated with influenza A (H1N1) in patients with severe acute respiratory illness. J. Clin. Virol. 2019; 116: 62-8. DOI: http://doi.org/10.1016/j.jcv.2019.05.003
- Fukunaga S., Ishida C., Nakaoka A., Ito T. A case of acute kidney injury and disseminated intravascular coagulation associated with influenza B viral infection. CEN Case Rep. 2014; 4(1): 95-100. DOI: http://doi.org/10.1007/s13730-014-0147-9
- Watanabe T., Yoshikawa H., Abe Y., Yamazaki S., Uehara Y., Abe T. Renal involvement in children with influenza A virus infection. Pediatr. Nephrol. 2003; 18(6): 541-4. DOI: http://doi.org/10.1007/s00467-003-1143-z
- Sellers S.A., Hagan R.S., Hayden F.G., Fischer W.A. The hidden burden of influenza: A review of the extra-pulmonary complications of influenza infection. Influenza Other Respir. Viruses. 2017; 11(5): 372-93. DOI: http://doi.org/10.1111/irv.12470
- Vardeny O., Solomon S.D. Influenza and hearth failure: a catchy comorbid combination. JACC: Hearth Fail. 2018; 7(2): 118-20. DOI: http://doi.org/10.1016/j.jchf.2018.11.008
- Pearce D.C., McCaw J.M., McVernon J., Mathews J.D. Influenza as a trigger for cardiovascular disease: An investigation of serotype, subtype and geographic location. Environ. Res. 2017; 156: 688-96. DOI: http://doi.org/10.1016/j.envres.2017.04.024
- Mamas M.A., Fraser D., Neyses L. Cardiovascular manifestations associated with influenza virus infection. Int. J. Cardiol. 2008; 130(3): 304-9.
- Vivaldi M.T., Kloner R.A., Schoen F.J. Triphenyltetrazolium staining of irreversible ischemic injury following coronary artery occlusion in rats. Am. J. Pathol. 1985; 121(3): 522-30.
- Bianchi G., Fox U., Imbaschiati E. Development of a strain of spontaneously hypertensive rat. Life Sciences. 1974; 14(2): 339-47.
- Doggrell S.A., Brown L. Rat models of hypertension, cardiac hypertrophy and failure. Cardiovascular Research. 1998; 39(1): 89- 105. DOI: http://doi.org/10.1016/s0008-6363(98)00076-5
- Daniels M.J., Selgrade M.K., Doerfler D., Gilmour M.I. Kinetic profile of influenza virus infection in three rat strains. Comp. Med. 2003; 53(3): 293-8.
- Цинзерлинг В.А., Воробьев С.Л., Зарубаев В.В., Дедов В.А., Беляевская С.В., Эсауленко Е.В. и др. Патогенетические аспекты гриппа в период эпидемии, вызванной вирусом A/ H1N1v в 2009-2010 гг., по аутопсии. Архив патологии. 2011; 73(6): 21-5.
- Taylor C.R., Levenson R.M. Quantification of immunohistochemistry – issues concerning methods, utility and semiquantitative assessment I. Histopathology. 2006; 49(4): 411-24. DOI: http://doi.org/10.1111/j.1365-2559.2006.02513.x
- Ibanes J.D., Morgan K.T., Burleson G.R. Histopathological changes in the upper respiratory tract of F344 rats following infection with a rat-adapted influenza virus. Vet. Pathol. 1996; 33(4): 412-8. DOI: http://doi.org/10.1177/030098589603300407