Kinetic Peculiarities of Nucleic Acid Elongation as a Multistage Cosequent Enzymatic Reaction

A. V. Lukovenkov; Луковенков А. В.; V. I. Bykov; Быков В. И.; S. D. Varfolomeev; Варфоломеев С. Д.

doi:10.31857/S0453881123020041

Kinetic Peculiarities of Nucleic Acid Elongation as a Multistage Cosequent Enzymatic Reaction

Authors: Lukovenkov A.V.¹, Bykov V.I.¹^,2, Varfolomeev S.D.¹^,2^,3
Affiliations:
1. Emanuel Institute of Biochemical Physics, Russian Academy of Sciences
2. Lomonosov Moscow State University, Institute of Physicochemical Foundations of the Functioning of Neural Network and Artificial Intellegence
3. Lomonosov Moscow State University, Faculty of Chemistry, Department of Chemical Ensymology
Issue: Vol 64, No 2 (2023)
Pages: 181-188
Section: ARTICLES
URL: https://journals.rcsi.science/0453-8811/article/view/137002
DOI: https://doi.org/10.31857/S0453881123020041
EDN: https://elibrary.ru/GNEEBJ
ID: 137002

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Abstract

The article investigates kinetics of nucleic acid elongation as a multistage consequent reaction curling up to cycle. There is represented a matematically precise proof of empiric formulae used to estimate elongation time depending on nucleotide chain length. There are given some estimations of characteristic elongation time for typical chain lengths, for example for new coronavirus (SARS-nCoV-2). There is also investigated stability of elongation kinetics and at typical chain lenghts is shown an existance of instable oscillating solution component in addition to main exponential component.

Keywords

nucleic acid elongation, polymerase chain reaction, kinetics of consequent reactions, multistage reactions, stability in chemnical kinetics

About the authors

A. V. Lukovenkov

Emanuel Institute of Biochemical Physics, Russian Academy of Sciences

Author for correspondence.
Email: luks.mipt@gmail.com
Russia, 119334, Moscow, Kosygina str., 4

V. I. Bykov

Email: luks.mipt@gmail.com
Russia, 119334, Moscow, Kosygina str., 4; Russia, 119192, Moscow, Lomonosovsky avenue, 27/1, of. E801-E804, A818

S. D. Varfolomeev

Emanuel Institute of Biochemical Physics, Russian Academy of Sciences; Lomonosov Moscow State University, Institute of Physicochemical Foundations of the Functioning of Neural Network
and Artificial Intellegence; Lomonosov Moscow State University, Faculty of Chemistry, Department of Chemical Ensymology

Email: luks.mipt@gmail.com
Russia, 119334, Moscow, Kosygina str., 4; Russia, 119192, Moscow, Lomonosovsky avenue, 27/1, of. E801-E804, A818; Russia, 119192, Moscow, Kolmogorova str., 1, building 11b

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