Approaches to standardization of common raspberry leaves (Rubus idaeus L.)
- Authors: Melnikov D.V.1, Braslavsky V.B.1, Kurkin V.A.1, Sazanova K.N.1, Ryzhov V.M.1
-
Affiliations:
- Samara State Medical University
- Issue: Vol 74, No 6 (2025)
- Pages: 25-31
- Section: Pharmaceutical chemistry and pharmacognosy
- URL: https://journals.rcsi.science/0367-3014/article/view/316989
- DOI: https://doi.org/10.29296/25419218-2025-06-03
- ID: 316989
Cite item
Open Access
Access granted
Subscription Access
Abstract
Introduction. Common raspberry (Rubus idaeus L.) is one of the representatives of the genus Rubus L., belonging to the Rosaceae family, which is widespread and cultivated in the Russian Federation. The fruits and leaves of common raspberry, due to their rich chemical composition (flavonoids, phenylpropanoids, saponins, organic acids and tannins), are used in folk practice as an anti-inflammatory, antipyretic and diaphoretic agent. Common raspberry leaf was included in the British Pharmacopoeia, but in the Russian Federation there is no regulatory documentation that regulates the quality of common raspberry leaves.
The aim of the work was to develop methods for the qualitative and quantitative determination of the amount of flavonoids in common raspberry leaves.
Material and methods. The object of the study is the leaves of common raspberry, harvested during the flowering phase in different areas of the Samara region in June – July 2024. Qualitative determination of flavonoids was carried out by thin-layer chromatography (TLC) using chromatographic carriers of Sorbfil brand plates (PTLC-AF-A-UV). To separate the substances, a solvent system was used: n-butyl alcohol – glacial acetic acid – water in a ratio of 4:1:2. Quantitative determination of the amount of flavonoids in aqueous-alcoholic extracts of common raspberry leaves was carried out by differential spectrophotometry on a Specord 40 device (AnalytikJena AG, Germany) calculated on standard sample of rutin.
Results and discussion. The developed method of quantitative determination was used to calculate the content of total flavonoids in common raspberry leaves, which varied from 2.08±0.06 to 2.11±0.06%. A validation assessment of the developed method was carried out. The error of a single determination with a confidence probability of 95% is ±2.86%.
Conclusion. A method of quantitative determination of total flavonoids in common raspberry leaves by differential spectrophotometry was developed. A method of qualitative determination of flavonoids in common raspberry leaves was developed using thin-layer chromatography in the solvent system n-butyl alcohol – glacial acetic acid – water in a ratio of (4:1:2) with a witness substance, a standard sample of rutin. The obtained research results can be used in the development of regulatory documentation for a promising type of raw material «Common raspberry leaves» for introduction into the State Pharmacopoeia of the Russian Federation.
Keywords
Full Text
##article.viewOnOriginalSite##About the authors
Danila Vadimovich Melnikov
Samara State Medical University
Author for correspondence.
Email: kvitravn@yandex-melnikov.ru
ORCID iD: 0009-0005-3294-326X
Postgraduate Student of the Department of Pharmacognosy with Botany and the Basics of Phytotherapy
Russian Federation, Chapaevskaya str., 89, Samara, 443099Valery Borisovich Braslavsky
Samara State Medical University
Email: v.b.braslavskii@samsmu.ru
ORCID iD: 0000-0002-6050-3377
SPIN-code: 7025-2239
Doctor of Pharmaceutical Sciences, Associate Professor, Associate Professor of the Department of Pharmacognosy with Botany and the Basics of Phytotherapy
Russian Federation, Chapaevskaya str., 89, Samara, 443099Vladimir Alexandrovich Kurkin
Samara State Medical University
Email: v.a.kurkin@samsmu.ru
ORCID iD: 0000-0002-7513-9352
SPIN-code: 1974-5537
Head of the Department of Pharmacognosy with Botany and the Basics of Phytotherapy, Doctor of Pharmaceutical Sciences, Professor
Russian Federation, Chapaevskaya str., 89, Samara, 443099Ksenia Nikolaevna Sazanova
Samara State Medical University
Email: k.n.sazanova@samsmu.ru
ORCID iD: 0000-0002-4287-8386
SPIN-code: 3867-6152
Candidate of Pharmaceutical Sciences, Senior Lecturer of the Department of Pharmacy Management and Economics – Basic Department «Pharmacies Plus»
Russian Federation, Chapaevskaya str., 89, Samara, 443099Vitaly Mikhailovich Ryzhov
Samara State Medical University
Email: v.m.ryzhov@samsmu.ru
ORCID iD: 0000-0002-8399-9328
SPIN-code: 4522-4652
Candidate of Pharmaceutical Sciences, Associate Professor, Associate Professor of the Department of Pharmacognosy with Botany and the Basics of Phytotherapy
Russian Federation, Chapaevskaya str., 89, Samara, 443099References
- Маевский П.Ф. Флора средней полосы европейской части России. 10-е издание, Москва: товарищество научных изданий КМК, 2006; 289–300. [Mayevsky P.F. Flora of the middle zone of the European part of Russia. 10th edition, Moscow: Association of Scientific Publications of the KMC, 2006; 289–300 (in Russian)]
- Гуляев Д.К., Белоногова В.Д., Рудакова И.П., Коротков И.В. Исследование полисахаридов побегов малины обыкновенной и их сорбционной активности. Человек и его здоровье. 2022; 25 (3): 72–80. [Gulyaev D.K., Belonogova V.D., Rudakova I.P., Korotkov I.V. Investigation of raspberry shoot polysaccharides and their sorption activity. Man and his health. 2022; 25 (3): 72–80 (in Russian)]
- Гуляев Д.К., Белоногова В.Д., Боков Д.О., Бессонов В.В. Антиоксидантная активность побегов первого года Rubus idaeus (L.). Вопросы обеспечения качества лекарственных средств. 2021; 31 (1): 16–21. [Gulyaev D.K., Belonogova V.D., Bokov D.O., Bessonov V.V. Antioxidant activity of the shoots of the first year of Rubus idaeus (L.). Issues of quality assurance of medicines. 2021; 31 (1): 16–21 (in Russian)]
- Дулов М.И., Антипенко М.И. Биохимический состав ягод сортов малины обыкновенной в условиях среднего Поволжья. Теоретические и прикладные проблемы агропромышленного комплекса. 2024; 59 (1): 37–42. [Dulov M.I., Antipenko M.I. Biochemical composition of raspberry varieties in the conditions of the Middle Volga region. Theoretical and applied problems of the agro-industrial complex. 2024; 59 (1): 37–42 (in Russian)]
- Растительные ресурсы СССР. Цветковые растения, их химический состав, использование. Семейства Hydrangeaceae – Haloragaceae. Л.: Наука, Ленинградское отделение, 1987; 326. [Plant resources of the USSR. Flowering plants, their chemical composition, use. Families Hydrangeaceae – Haloragaceae. L.: Nauka, Leningrad branch, 1987; 326 (in Russian)]
- Растительные ресурсы России: Дикорастущие цветковые растения, их компонентный состав и биологическая активность. Т. 2. Семейства Actinidiaceae, Malvaceae – Euphorbiaceae, Haloragaceae. Отв. редактор А.Л. Буданцев. СПб.; М.: Товарищество научных изданий КМК, 2009; 513. [Plant resources of Russia: Wild flowering plants, their component composition and biological activity. Vol. 2. Families Actinidiaceae, Malvaceae – Euphorbiaceae, Haloragaceae. Responsible editor A.L. Budantsev. St. Petersburg; Moscow: Partnership of scientific publications KMK, 2009; 513 (in Russian)]
- British Pharmacopoeia. 4th Ed. Rockville: British Pharmacopoeia Inc. 2022; 437–8. [Британская фармакопея. 4-е Изд. Роквилл: Британская Фармакопея. Inc. 2022; 437–8]
- Губанов И.А., Киселева К.В., Новиков В.С., Тихомиров В.Н. М.: Товарищество науч. изд. КМК: Ин-т технол. исслед. Иллюстрированный определитель растений Средней России. Покрытосеменные (двудольные: раздельнолепестные). 2003; 2: 402–8. [Gubanov I.A., Kiseleva K.V., Novikov V.S., Tikhomirov V.N. Moscow: Comradeship scientific publishing House. KMK: In-t technol. issledovanie. Illustrated determinant of plants of Central Russia. Angiosperms (Dicotyledons: deciduous). 2003; 2: 402–8 (in Russian)]
- Государственная Фармакопея Российской Федерации, XV изд. [Электронное издание]. Режим доступа: https://pharmacopoeia.regmed.ru/ [Дата обращения ХХ Декабря, 2024]. [The State Pharmacopoeia of The Russian Federation, XV ed. [Electronic edition]. Access mode: https://pharmacopoeia.regmed.ru/ [Accessed 20 December, 2024] (in Russian)]
Supplementary files
