Effect of Short-term Dry Immersion on Proteolytic Signaling in the Human Soleus Muscle
- Authors: Vil’chinskaya N.A.1, Mirzoev T.M.1, Lomonosova Y.N.1, Kozlovskaya I.B.1, Shenkman B.S.1
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Affiliations:
- Institute of Biomedical Problems
- Issue: Vol 43, No 7 (2017)
- Pages: 787-792
- Section: Article
- URL: https://journals.rcsi.science/0362-1197/article/view/177388
- DOI: https://doi.org/10.1134/S0362119717070209
- ID: 177388
Cite item
Abstract
We analyzed the signaling processes initiating proteolytic events in the human soleus muscle during short-term exposure under the non-weight-bearing conditions. Dry immersion (DI) was used to induce weight deprivation in the m. soleus for 3 days. Western blotting was used to determine the level of insulin receptor substrate 1 (IRS-1), total and phosphorylated neuronal NO synthase (nNOS), and adenosine monophosphate-activated protein kinase (AMPK), which control the anabolic and catabolic signaling pathways, and the level of cytoskeletal protein desmin and Са2+-activated protease calpain. By day 3 of DI, calpain- dependent proteolysis manifests itself by reductions in both the total content and level of nNOS phosphorilation. The rate of AMPK phosphorylation was significantly decreased.
About the authors
N. A. Vil’chinskaya
Institute of Biomedical Problems
Author for correspondence.
Email: vilchinskaya2008@rambler.ru
Russian Federation, Moscow, 123007
T. M. Mirzoev
Institute of Biomedical Problems
Email: vilchinskaya2008@rambler.ru
Russian Federation, Moscow, 123007
Yu. N. Lomonosova
Institute of Biomedical Problems
Email: vilchinskaya2008@rambler.ru
Russian Federation, Moscow, 123007
I. B. Kozlovskaya
Institute of Biomedical Problems
Email: vilchinskaya2008@rambler.ru
Russian Federation, Moscow, 123007
B. S. Shenkman
Institute of Biomedical Problems
Email: vilchinskaya2008@rambler.ru
Russian Federation, Moscow, 123007