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The double toxic MPTP + CBE presymptomatic Parkinson-like phenotype in mice
- Authors: Pchelina S.N.1,2, Bezrukova A.I.1,2, Rudenok M.M.3, Zhuravlev A.S.1,2, Rybolovlev I.N.3, Lavrinova A.O.1,2, Baydakova G.V.4, Nikolaev M.A.1,2, Nesterov M.S.5, Abaimov D.A.6, Pidurchina V.N.1, Partevyan S.A.3, Semenova E.I.3, Usenko T.S.1,2, Zakharova E.Y.3, Emelyanov A.K.1,2, Shadrina M.I.3, Slominsky P.A.3
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Affiliations:
- Petersburg Nuclear Physics Institute, Kurchatov Institute National Research Center
- Pavlov First Saint Petersburg State Medical University
- Institute of Molecular Genetics, Kurchatov Institute National Research Centre
- Research Center of Medical Genetics
- Scientific Center of Biomedical Technologies, Federal Medical and Biological Agency of Russia
- Research Centre of Neurology
- Issue: Vol 90, No 8 (2025)
- Pages: 1148-1162
- Section: Articles
- URL: https://journals.rcsi.science/0320-9725/article/view/356271
- DOI: https://doi.org/10.31857/S0320972525080065
- EDN: https://elibrary.ru/VCATHO
- ID: 356271
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Abstract
The deficiency of glucocerebrosidase (GCase) encoded by the GBA1 gene, leads to the autosomal recessive Gaucher disease and highly increased risk of developing Parkinson's disease (PD). In order to study the effect of GCase dysfunction on neurodegeneration, we evaluated the GCase activity, lysosphingolipid content, extent of dopaminergic neuron degeneration in the substantia nigra (SN), and levels of dopamine (DA) and total and oligomeric α-synuclein (α-Syn) in the brain of mice with the presymptomatic stage of parkinsonism induced by 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) in combination with a single injection of the GCase selective inhibitor conduritol-β-epoxide (CBE) (100 mg/kg body weight). A single injection of CBE led to a ~50% decrease in the GCase activity, significant increase in the lysosphingolipid content, and striatal accumulation of oligomeric α-Syn in the mouse brain. Assessment of the DA neuron degeneration in the SN 14 days after injection by immunohistochemical staining for tyrosine hydroxylase (TH) demonstrated a twice more pronounced reduction in the number of TH+ neurons in MPTP + CBE mice compared to MPTP only-treated animals (14% vs. 29%, respectively; p < 0.0001). The double neurotoxic (MPTP+CBE) model was also characterized by a decrease in the DA content and more pronounced accumulation of total α-Syn in the striatum. Overall, we demonstrated that inhibition of the GCase activity leads to the α-Syn accumulation and further exacerbation of the MPTP-induced pathology. The described double toxic MPTP + CBE mouse model can be used for the screening of neuroprotective drugs in approaches aimed at increasing the GCase activity.
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About the authors
S. N. Pchelina
Petersburg Nuclear Physics Institute, Kurchatov Institute National Research Center; Pavlov First Saint Petersburg State Medical University
Author for correspondence.
Email: sopchelina@hotmail.com
Gatchina; Saint Petersburg
A. I. Bezrukova
Petersburg Nuclear Physics Institute, Kurchatov Institute National Research Center; Pavlov First Saint Petersburg State Medical University
Email: sopchelina@hotmail.com
Gatchina; Saint Petersburg
M. M. Rudenok
Institute of Molecular Genetics, Kurchatov Institute National Research Centre
Email: sopchelina@hotmail.com
Moscow
A. S. Zhuravlev
Petersburg Nuclear Physics Institute, Kurchatov Institute National Research Center; Pavlov First Saint Petersburg State Medical University
Email: sopchelina@hotmail.com
Gatchina; Saint Petersburg
I. N. Rybolovlev
Institute of Molecular Genetics, Kurchatov Institute National Research Centre
Email: sopchelina@hotmail.com
Moscow
A. O. Lavrinova
Petersburg Nuclear Physics Institute, Kurchatov Institute National Research Center; Pavlov First Saint Petersburg State Medical University
Email: sopchelina@hotmail.com
Gatchina; Saint Petersburg
G. V. Baydakova
Research Center of Medical Genetics
Email: sopchelina@hotmail.com
Moscow
M. A. Nikolaev
Petersburg Nuclear Physics Institute, Kurchatov Institute National Research Center; Pavlov First Saint Petersburg State Medical University
Email: sopchelina@hotmail.com
Gatchina; Saint Petersburg
M. S. Nesterov
Scientific Center of Biomedical Technologies, Federal Medical and Biological Agency of Russia
Email: sopchelina@hotmail.com
Moscow
D. A. Abaimov
Research Centre of Neurology
Email: sopchelina@hotmail.com
Moscow
V. N. Pidurchina
Petersburg Nuclear Physics Institute, Kurchatov Institute National Research Center
Email: sopchelina@hotmail.com
Gatchina
S. A. Partevyan
Institute of Molecular Genetics, Kurchatov Institute National Research Centre
Email: sopchelina@hotmail.com
Moscow
E. I. Semenova
Institute of Molecular Genetics, Kurchatov Institute National Research Centre
Email: sopchelina@hotmail.com
Moscow
T. S. Usenko
Petersburg Nuclear Physics Institute, Kurchatov Institute National Research Center; Pavlov First Saint Petersburg State Medical University
Email: sopchelina@hotmail.com
Gatchina; Saint Petersburg
E. Y. Zakharova
Institute of Molecular Genetics, Kurchatov Institute National Research Centre
Email: sopchelina@hotmail.com
Moscow
A. K. Emelyanov
Petersburg Nuclear Physics Institute, Kurchatov Institute National Research Center; Pavlov First Saint Petersburg State Medical University
Email: sopchelina@hotmail.com
Gatchina; Saint Petersburg
M. I. Shadrina
Institute of Molecular Genetics, Kurchatov Institute National Research Centre
Email: sopchelina@hotmail.com
Moscow
P. A. Slominsky
Institute of Molecular Genetics, Kurchatov Institute National Research Centre
Email: sopchelina@hotmail.com
Moscow
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