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R and S-Pyruvate-Containing Polysaccharide from the Cell Wall of Rathayibacter sp. VKM AC-2927
- Authors: Kokoulin M.S.1, Potekhina N.V.2, Tul’skaya E.M.2, Ospennikov Y.V.3, Evtushenko L.I.3
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Affiliations:
- Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch, Russian Academy of Sciences
- Faculty of Biology, Lomonosov Moscow State University
- All-Russian Collection of Microorganisms (VKM), Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences
- Issue: Vol 90, No 4 (2025)
- Pages: 550-558
- Section: Articles
- URL: https://journals.rcsi.science/0320-9725/article/view/312338
- DOI: https://doi.org/10.31857/S0320972525040049
- EDN: https://elibrary.ru/ihuojn
- ID: 312338
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Abstract
The structures of two cell wall glycopolymers from the strainRathayibacter sp. VKM Ac-2927 (family Microbacteriaceae, class Actinomycetes), isolated from linden leaves affected by leaf miner, were established using chemical and NMR spectroscopy methods. The first polymer, rhamnomannan, is a heterogeneous chain polymer composed of regularly alternating α-D-rhamnose and α-D-mannose residues linked by (1→3)- and (1→2)-glycosidic bonds and contains a minor amount of lateral β-D-xylopyranose residues (the structure is presented in the text). The second polymer, acetalized withR- andS-pyruvic acid, consists of branched tetrasaccharide units, →3)-α-D-Galp4,6-(R-Pyr)-(1→3)-β-D-Glcp-(1→6)-[β-D-Galp3,4-(S-Pyr)-(1→4)]-α-D-Manp-(1→. The structure of this polysaccharide is new for representatives of the genusRathayibacterand prokaryotes in general. The results of the present study indicate the structural diversity of microbial glycopolymers and are consistent with previously obtained data on the specificity of their composition for species of the genus Rathayibacter.
About the authors
M. S. Kokoulin
Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch, Russian Academy of Sciences690022 Vladivostok, Russia
N. V. Potekhina
Faculty of Biology, Lomonosov Moscow State University
Email: potekhina@mail.ru
119234 Moscow, Russia
E. M. Tul’skaya
Faculty of Biology, Lomonosov Moscow State University119234 Moscow, Russia
Y. V. Ospennikov
All-Russian Collection of Microorganisms (VKM), Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences142290 Pushchino, Moscow Region, Russia
L. I. Evtushenko
All-Russian Collection of Microorganisms (VKM), Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences142290 Pushchino, Moscow Region, Russia
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