A solitary stalled 80S ribosome prevents mRNA recruitment to stress granules

Мұқаба

Дәйексөз келтіру

Толық мәтін

Ашық рұқсат Ашық рұқсат
Рұқсат жабық Рұқсат берілді
Рұқсат жабық Тек жазылушылар үшін

Аннотация

In response to stress stimuli, eukaryotic cells typically suppress protein synthesis. This leads to the mRNA release from polysomes, their condensation with RNA-binding proteins, and the formation of non-membrane-bound cytoplasmic compartments called stress granules (SGs). SGs contain 40S but generally lack 60S ribosomal subunits. It is known that cycloheximide, emetine, and anisomycin, the ribosome inhibitors which block the 80S ribosome progression along mRNA and stabilize polysomes, prevent SG assembly. Conversely, puromycin, which induces premature termination, releases mRNAs from polysomes and stimulates the formation of SGs. The same effect is caused by some translation initiation inhibitors, which lead to polysome disassembly and the accumulation of mRNAs in a form of stalled 48S preinitiation complexes. Based on these and other data, it is believed that the trigger for SG formation is the presence of mRNA with extended ribosome-free segments, which tend to form condensates in the cell. In this study, we evaluated the ability of various small-molecule translation inhibitors to block or stimulate the assembly of SGs under conditions of severe oxidative stress induced by sodium arsenite. Contrary to expectations, we found that ribosome-targeting elongation inhibitors of a specific type, that arrest solitary 80S ribosomes at the beginning of the mRNA coding regions but do not interfere with all subsequent ribosomes in completing translation and leaving the transcripts (such as harringtonine, lactimidomycin, or T-2 toxin), completely prevented the formation of arsenite-induced SGs. These observations suggest that the presence of even a single 80S ribosome on mRNA is sufficient to prevent its recruitment into SGs, and the presence of extended ribosome-free regions of mRNA is not sufficient for SG formation. We propose that mRNA entry into SGs may be mediated by specific contacts between RNA-binding proteins and those regions on 40S subunits that remain inaccessible when ribosomes are associated.

Авторлар туралы

A. Fedorovskiy

Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University;Faculty of Materials Science, Lomonosov Moscow State University

119234 Moscow, Russia;119234 Moscow, Russia

A. Burakov

Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University

119234 Moscow, Russia

I. Terenin

Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University;Sirius University of Science and Technology

119234 Moscow, Russia;354340 Sirius, Krasnodar region, Russia

D. Bykov

Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University;Faculty of Biology, Lomonosov Moscow State University;Engelhardt Institute of Molecular Biology, Russian Academy of Sciences

119234 Moscow, Russia;119234 Moscow, Russia;119991 Moscow, Russia

K. Lashkevich

Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University

119234 Moscow, Russia

V. Popenko

Engelhardt Institute of Molecular Biology, Russian Academy of Sciences

119991 Moscow, Russia

N. Makarova

Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University

119234 Moscow, Russia

I. Sorokin

Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University

119234 Moscow, Russia

A. Sukhinina

Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University;Faculty of Bioengineering and Bioinformatics, Lomonosov Moscow State University

119234 Moscow, Russia;119234 Moscow, Russia

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