Diagnostic significance of determining the expression of energy metabolism genes in fetal growth retardation
- 作者: Kan N.E.1, Soldatova E.E.1, Tyutyunnik V.L.1, Volochaeva M.V.1, Sadekova A.A.1, Krasnyi A.M.1
-
隶属关系:
- Academician V.I. Kulakov National Medical Research Centre for Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia
- 期: 编号 8 (2023)
- 页面: 48-55
- 栏目: Original Articles
- URL: https://journals.rcsi.science/0300-9092/article/view/274641
- DOI: https://doi.org/10.18565/aig.2023.93
- ID: 274641
如何引用文章
开放存取
##reader.subscriptionAccessGranted##
订阅存取
详细
Objective: To determine the level of expression of energy metabolism genes, namely visfatin (NAMPT), ghrelin (GHRL) and leptin (LEP) in maternal and umbilical cord blood and in the placenta in case of fetal growth retardation.
Materials and methods: The study included 52 pregnant women: the main group consisted of 27 patients diagnosed with fetal growth retardation postnatally; the control group included 25 women with normal course of pregnancy. Real-time PCR was used to determine the expression level of energy metabolism genes.
Results: The level of expression of the NAMPT and GHRL genes in maternal blood was found to be statistically significantly reduced in fetal growth retardation (p=0.012 and p=0.019, respectively). The level of expression of the NAMPT and GHRL genes in umbilical cord blood was also reduced in comparison with the control group, but it was not statistically significant (p=0.30 and p=0.23, respectively). LEP gene expression in maternal and umbilical cord blood was not found. The level of leptin expression in the placenta was found to be statistically significantly increased in the main group (p=0.045), though these differences were not associated with gestational age at the time of delivery.
Conclusion: The decreased levels of expression of the NAMPT and GHRL genes in maternal blood can become an objective marker for diagnosing fetal growth retardation during pregnancy. The increased expression of LEP in the placenta in fetal growth retardation may give a better understanding of its pathogenesis and new opportunities for its diagnosis.
作者简介
Natalia Kan
Academician V.I. Kulakov National Medical Research Centre for Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia
Email: kan-med@mail.ru
ORCID iD: 0000-0001-5087-5946
SPIN 代码: 5378-8437
Scopus 作者 ID: 57008835600
Researcher ID: B-2370-2015
Dr. Med. Sci., Professor, MD, PhD, Deputy Director of Science
俄罗斯联邦, MoscowEkaterina Soldatova
Academician V.I. Kulakov National Medical Research Centre for Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia
编辑信件的主要联系方式.
Email: katerina.soldatova95@bk.ru
ORCID iD: 0000-0001-6463-3403
Postgraduate Student
俄罗斯联邦, MoscowVictor Tyutyunnik
Academician V.I. Kulakov National Medical Research Centre for Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia
Email: tioutiounnik@mail.ru
ORCID iD: 0000-0002-5830-5099
SPIN 代码: 1963-1359
Scopus 作者 ID: 56190621500
Researcher ID: B-2364-2015
Professor, MD, PhD, Leading Researcher of Center of Scientific and Clinical Researches
俄罗斯联邦, MoscowMaria Volochaeva
Academician V.I. Kulakov National Medical Research Centre for Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia
Email: m_volochaeva@oparina4.ru
ORCID iD: 0000-0001-8953-7952
PhD, Senior Researcher, Department of Regional Cooperation and Integration, Physician at the 1st Maternity Department
俄罗斯联邦, MoscowAlsu Sadekova
Academician V.I. Kulakov National Medical Research Centre for Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia
Email: a_sadekova@oparina4.ru
ORCID iD: 0000-0003-4726-7477
PhD, Researcher at the Cytology Laboratory
俄罗斯联邦, MoscowAleksey Krasnyi
Academician V.I. Kulakov National Medical Research Centre for Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia
Email: alexred@list.ru
ORCID iD: 0000-0001-7883-2702
PhD (Bio), Head of the Cytology Laboratory
俄罗斯联邦, Moscow参考
- Gluckman P.D., Hanson M.A., Pinal C. The developmental origins of adult disease. Matern. Child Nutr. 2005; 1(3): 130-41. https://dx.doi.org/10.1111/j.1740-8709.2005.00020.x.
- Леонова И. А., Иванов Д. О. Фетальное программирование и ожирение у детей. Детская медицина Северо-Запада 2015; 6(3): 28-41. [Leonova I.A., Ivanov D.O. Fetal programming and obesity in children. Children's Medicine of the North-West. 2015; 6(3): 28-41. (in Russian)].
- Железова М.Е., Зефирова Т.П., Канюкова С.С. Задержка роста плода: современные подходы к диагностике и ведению беременности. Практическая медицина. 2019; 17(4): 8-14. [Zhelezova M.E., Zefirova T.A., Kanyukov S.S. Fetal growth restriction: modern approaches to the diagnosis and management of pregnancy. Practical Medicine. 2019; 17(4): 8-14. (in Russian)]. https://dx.doi.org/10.32000/2072-1757-2019-4-8-14.
- Dessì A., Pravettoni C., Cesare Marincola F., Schirru A., Fanos V. The biomarkers of fetal growth in intrauterine growth retardation and large for gestational age cases: from adipocytokines to a metabolomic all-in-one tool. Expert Rev. Proteomics. 2015; 12(3): 309-16. https://dx.doi.org/10.1586/ 14789450.2015.1034694.
- Кан Н.Е., Тютюнник В.Л., Хачатрян З.В., Садекова А.А., Красный А.М. Метилирование генов TLR2 и импринтинг-контролирующей области IGF2/H19 в плазме крови при задержке роста плода. Акушерство и гинекология. 2021; 5: 79-84. [Kan N.E., Tyutyunnik V.L., Khachatryan Z.V., Sadekova A.A., Krasnyi A.M. Methylation of the TLR2 genes and the IGF2/H19 imprinting-control region in blood plasma in fetal growth retardation. Obstetrics and Gynecology. 2021; (5): 79-84. (in Russian)]. https://dx.doi.org/10.18565/aig.2021.5.79-84.
- Cekmez F., Canpolat F.E., Pirgon O., Aydemir G., Tanju I.A., Genc F.A. et al. Adiponectin and visfatin levels in extremely low birth weight infants; they are also at risk for insulin resistance. Eur. Rev. Med. Pharmacol. Sci. 2013; 17(4): 501-6.
- Lee M.H., Jeon Y.J., Lee S.M., Park M.H., Jung S.C., Kim Y.J. Placental gene expression is related to glucose metabolism and fetal cord blood levels of insulin and insulin-like growth factors in intrauterine growth restriction. Early Hum. Dev. 2010; 86(1): 45-50. https://dx.doi.org/10.1016/ j.earlhumdev.2010.01.001.
- Maymó J.L., Pérez Pérez A., Gambino Y., Calvo J.C., Sánchez-Margalet V., Varone C.L. Review: Leptin gene expression in the placenta--regulation of a key hormone in trophoblast proliferation and survival. Placenta. 2011; 32(Suppl. 2): S146-53. https://dx.doi.org/10.1016/ j.placenta.2011.01.004.
- Morgan S.A., Bringolf J.B., Seidel E.R. Visfatin expression is elevated in normal human pregnancy. Peptides. 2008; 29(8): 1382-9. https://dx.doi.org/10.1016/ j.peptides.2008.04.010.
- Mazaki-Tovi S., Romero R., Kusanovic J.P., Vaisbuch E., Erez O., Than N.G. et al. Maternal visfatin concentration in normal pregnancy. J. Perinat. Med. 2009; 37(3): 206-17. https://dx.doi.org/10.1515/JPM.2009.054.
- Briana D.D., Malamitsi-Puchner A. The role of adipocytokines in fetal growth. Ann. N. Y. Acad. Sci. 2010; 1205: 82-7. https://dx.doi.org/10.1111/ j.1749-6632.2010.05650.x.
- Moschen A.R., Kaser A., Enrich B., Mosheimer B., Theurl M., Niederegger H., Tilg H. Visfatin, an adipocytokine with proinflammatory and immunomodulating properties. J. Immunol. 2007; 178(3): 1748-58. https://dx.doi.org/10.4049/jimmunol.178.3.1748.
- Pavlová T., Novák J., Bienertová-Vašků J. The role of visfatin (PBEF/Nampt) in pregnancy complications. J. Reprod. Immunol. 2015; 112: 102-10. https://dx.doi.org/10.1016/j.jri.2015.09.004.
- Martín-Estal I., de la Garza R.G., Castilla-Cortázar I. Intrauterine growth retardation (IUGR) as a novel condition of insulin-like growth factor-1 (IGF-1) deficiency. Rev. Physiol. Biochem. Pharmacol. 2016; 170: 1-35. https://dx.doi.org/10.1007/112_2015_5001.
- Krasnyi A.M., Sadekova A.A., Smolnova T.Y., Chursin V.V., Buralkina N.A., Chuprynin V.D., Yarotskaya E., Pavlovich S.V.., Sukhikh G.T. The levels of Ghrelin, Glucagon, Visfatin and Glp-1 Are Decreased in the Peritoneal Fluid of women with endometriosis along with the increased expression of the CD10 protease by the macrophages. Int. J. Mol. Sci. 2022; 23(18): 10361. https://dx.doi.org/ 10.3390/ijms2318103611.
- Barrientos G., Toro A., Moschansky P., Cohen M., Garcia M.G., Rose M. et al. Leptin promotes HLA-G expression on placental trophoblasts via the MEK/Erk and PI3K signaling pathways. Placenta. 2015; 36(4): 419-26. https://dx.doi.org/10.1016/j.placenta.2015.01.006.
- Stefaniak M., Dmoch-Gajzlerska E. Maternal serum and cord blood leptin concentrations at delivery in normal pregnancies and in pregnancies complicated by intrauterine growth restriction. Obes. Facts. 2022; 15(1): 62-9. https://dx.doi.org/10.1159/000519609.
- Karakosta P., Roumeliotaki T., Chalkiadaki G., Sarri K., Vassilaki M., Venihaki M. et al. Cord blood leptin levels in relation to child growth trajectories. Metabolism. 2016; 65(6): 874-82. https://dx.doi.org/10.1016/j.metabol.2016.03.003.
补充文件
