Targeting Liposomes Loaded with DNA Mimetics for the Directional Elimination of Tumor Cells
- 作者: Shramova E.1, Proshkina G.1, Deyev S.1,2
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隶属关系:
- Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences
- Sechenov First Moscow State Medical University of the Ministry of Health of the Russian Federation (Sechenov University)
- 期: 卷 40, 编号 1 (2023)
- 页面: 66-70
- 栏目: Articles
- URL: https://journals.rcsi.science/0233-4755/article/view/135027
- DOI: https://doi.org/10.31857/S0233475523010097
- EDN: https://elibrary.ru/NTWQTL
- ID: 135027
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详细
The article presents an innovative method for the targeted elimination of tumor cells of a certain molecular profile by inactivating the transcription of genes of common subunits of human RNA polymerases using complementary DNA mimetics delivered into cells inside liposomes modified on the outer surface with targeting molecules. It was shown that inactivation of genes of RNA polymerase common subunits Rpb5, Rpb6, or Rpb8 by the proposed method, depending on the chosen target, causes death of up to 50% of HER2-positive human breast cancer cells in culture.
作者简介
E. Shramova
Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences
编辑信件的主要联系方式.
Email: shramova.e.i@gmail.com
Russia, 117997, Moscow
G. Proshkina
Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences
Email: shramova.e.i@gmail.com
Russia, 117997, Moscow
S. Deyev
Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences; Sechenov First Moscow State Medical University of the Ministry of Health of the Russian Federation (Sechenov University)
Email: shramova.e.i@gmail.com
Russia, 117997, Moscow; Russia, 119991, Moscow
参考
- Werner F., Grohmann D. 2011. Evolution of multisubunit RNA polymerases in the three domains of life. Nat. Rev. Microbiol. 9, 85–98.
- Cuevas-Bermúdez A., Martínez-Fernández V., Garrido-Godino A.I., Navarro F. 2017. Subunits common to RNA polymerases. In: The Yeast Role in Medical Applications. Ed Abdulkhair W. M. H. London: IntechOpen, p. 151–165. https://doi.org/10.5772/intechopen.70936
- Zaros C., Briand J.F., Boulard Y., Labarre-Mariotte S., Garcia-Lopez M.C., Thuriaux P., Navarro F. 2007. Functional organization of the Rpb5 subunit shared by the three yeast RNA polymerases. Nucl. Acids Research. 35 (2), 634–647.
- Garrido-Godino A.I., Garcia-Lopez M.C., Navarro F. 2013. Correct assembly of RNA polymerase II depends on the foot domain and is required for multiple steps of transcription in Saccharomyces cerevisiae. Mol. Cell. Biol. 33, 3611–3626.
- Woychik N.A., Liao S.-M., Kolodziej P.A., Young R.A. 1990. Subunits shared by eukaryotic nuclear RNA polymerases. Genes Dev. 4, 313–323.
- Stahel R.A., Zangemeister-Wittke U. 2003. Antisense oligonucleotides for cancer therapy-an overview. Lung Cancer. 41 (Suppl 1), S81–S88.https://doi.org/10.1016/s0169-5002(03)00147-8
- Cohen J.S. 1993. Phosphorothioate oligonucleotides. In: Antisense Research and Applications. Eds Crooke S.T., Lebleu B. Florida: CRC Press, p. 205–223.
- Wengel J. 2001. LNA (locked nucleic acid). In: Antisense Drug Technology; Principles, Strategies, and Applications. Ed. Crooke, S.T. New York: Marcel Dekker, p. 339–357.
- Hagedorn P.H., Persson R., Funder E.D., Albæk N., Diemer S.L., Hansen D.J., Møller M.R., Papargyri N., Christiansen H., Hansen B.R., Hansen H.F., Jensen M.A., Koch T. 2018. Locked nucleic acid: Modality, diversity, and drug discovery. Drug Discov. Today. 23 (1), 101–114.
- Deyev S., Proshkina G., Baryshnikova O., Ryabova A., Avishai G., Katrivas L., Giannini C., Levi-Kalisman Y., Kotlyar A. 2018. Selective staining and eradication of cancer cells by protein-carrying DARPin-functionalized liposomes. Eur. J. Pharm. Biopharm. 130, 296–305.
- Shramova E.I., Shilova M.V., Ryabova A.V., Dzhalilova D.S., Zolotova N.A., Telegin G.B., Deyev S.M., Proshkina G.M. 2021. Barnase*Barstar-guided two-step targeting approach for drug delivery to tumor cells in vivo. J. Control. Release. 340, 200–208. https://doi.org/10.1016/j.jconrel.2021.11.001
- Mosmann T. 1983. Rapid colorimetric assay for cellular growth and survival: Application to proliferation and cytotoxicity assays. J. Immunol. Methods. 65 (1–2), 55–63.
- Steiner D., Forrer P., Plückthun A. 2008. Efficient selection of DARPins with sub-nanomolar affinities using SRP phage display. J. Mol. Biol. 382 (5), 1211–1227. https://doi.org/10.1016/j.jmb.2008.07.085
- Shilova O., Shramova E., Proshkina G., Deyev S. 2021. Natural and designed toxins for precise therapy: Modern approaches in experimental oncology. Int. J. Mol. Sci. 22 (9), 4975. https://doi.org/10.3390/ijms22094975
- Tolmachev V.M., Chernov V.I., Deyev S.M. 2022. Targeted nuclear mediine. Seek and destroy. Russ. Chem. Rev. 91, RCR5034. https://doi.org/10.1070/RCR5034
