Assessment of the Toxic Effect of 2-(Chlorodinitromethyl)-4-Methoxy-6-(4-Methylpiperazine-1-yl)-1,3,5-Triazine by Respiratory Activity of Lymphocytes

P. V. Iliasov; Ильясов П. В.; L. V. Limareva; Лимарева Л. В.; A. I. Sizova; Сизова А. И.; V. A. Zalomlenkov; Заломленков В. А.; A. P. Kuricyna; Курицына А. П.

doi:10.31857/S0233475523050055

Assessment of the Toxic Effect of 2-(Chlorodinitromethyl)-4-Methoxy-6-(4-Methylpiperazine-1-yl)-1,3,5-Triazine by Respiratory Activity of Lymphocytes

Authors: Iliasov P.V.¹, Limareva L.V.¹, Sizova A.I.¹, Zalomlenkov V.A.², Kuricyna A.P.¹
Affiliations:
1. Samara State Medical University, Ministry of Health of the Russian Federation
2. Samara State Technical University
Issue: Vol 40, No 5 (2023)
Pages: 342-350
Section: Articles
URL: https://journals.rcsi.science/0233-4755/article/view/135040
DOI: https://doi.org/10.31857/S0233475523050055
EDN: https://elibrary.ru/OETRRZ
ID: 135040

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Abstract

A method for evaluation of metabolic characteristics of intact cells based on electrochemical registration of their respiratory activity was used to monitor a reaction of lymphocytes to a potential pharmacological agent, 2-(chlorodinitromethyl)-4-methoxy-6-(4-methylpiperazin-1-yl)-1,3,5-triazine. The method ensured an estimation of cytotoxicity of the test compound and made it possible to determine its minimum toxic concentrations for human lymphocytes. It was shown that the obtained results agree with the data of a reference method – MTT-based cell viability assay.

Keywords

cytotoxicity, respiratory activity, lymphocytes, antitumor agent, Clark-type electrode, triazines

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