Study of the Protein Composition of Dry Blood Spots of Healthy Volunteers in the Experiment with Hypomagnetic Conditions

D. N. Kashirina; Каширина Д. Н.; I. M. Larina; Ларина И. М.; E. N. Nikolaev; Николаев Е. Н.; M. G. Tyuzhin; Тюжин М. Г.; O. V. Popova; Попова О. В.; V. Yu. Kukanov; Куканов В. Ю.; V. B. Rusanov; Русанов В. Б.; A. S. Kononikhin; Кононихин А. С.; A. G. Brzhozovskiy; Бржозовский А. Г.; L. Kh. Pastushkova; Пастушкова Л. Х.; O. I. Orlov; Орлов О. И.

doi:10.31857/S0131164622600483

Study of the Protein Composition of Dry Blood Spots of Healthy Volunteers in the Experiment with Hypomagnetic Conditions

作者: Kashirina D.N.¹, Larina I.M.¹, Nikolaev E.N.², Tyuzhin M.G.¹, Popova O.V.¹, Kukanov V.Y.¹, Rusanov V.B.¹, Kononikhin A.S.¹^,2, Brzhozovskiy A.G.¹^,2, Pastushkova L.K.¹, Orlov O.I.¹
隶属关系:
1. Institute of Biomedical Problems of the RAS
2. Skolkovo Institute of Science and Technology
期: 卷 49, 编号 1 (2023)
页面: 104-115
栏目: Articles
URL: https://journals.rcsi.science/0131-1646/article/view/139818
DOI: https://doi.org/10.31857/S0131164622600483
EDN: https://elibrary.ru/APZSPM
ID: 139818

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详细

Hypomagnetic conditions will be an integral part of the complex of factors affecting cosmonauts in long-term interplanetary missions outside the Earth’s magnetic field. Adaptation of the human body under these conditions will affect the regulatory processes in various physiological systems, the molecular pathways of which, with the participation of proteins, have not yet been studied. In order to fill this gap, dry blood spots collected on a special paper from healthy volunteers were studied by proteomic methods in two sessions of a model experiment: with hypomagnetic conditions and in a “placebo” session. The experiment was carried out by double-blind control, with the participation of the same volunteers in both sessions. 1219 different proteins were semiquantitatively determined in the samples. In the hypomagnetic conditions series, changes in three proteins: tropomyosin alpha-3 chain (TPM3), abhydrolase domain-containing protein 14B (ABHD14B), and acetylcholinesterase-associated protein (CUTA), relative to individual values before the experiment series were detected. However, comparison of the data obtained in the “placebo” session smoothed out the effect of hypomagnetic conditions on the changes in the aforementioned proteins. The results obtained, in our opinion, may either mean the absence of the influence of hypomagnetic conditions with a short exposure and the absence of a cumulative effect, or they are unreliable due to the insufficient number of subjects in the sample.

关键词

hypomagnetic conditions, healthy volunteers, proteomics, chromato-mass spectrometry, dry blood spots.

参考

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