Study of RNA polymerase transcription through nucleosome using the cryo-electron microscopy approach
- Авторы: Volokh O.I.1, Hsieh F.K.2, Karlova M.G.1, Trifonova E.S.1, Studitsky V.M.3, Sokolova O.S.1
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Учреждения:
- Department of Bioengineering, School of Biology
- Robert Wood Johnson Medical School
- Cancer Epigenetics Team
- Выпуск: Том 71, № 1 (2016)
- Страницы: 34-38
- Раздел: Molecular Biology
- URL: https://journals.rcsi.science/0096-3925/article/view/173467
- DOI: https://doi.org/10.3103/S0096392516010120
- ID: 173467
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Аннотация
A promising and reliable method for the analysis of macromolecule structures is capturing images using cryo-electron transmission microscopy and consequently performing three-dimensional reconstructions. In this study, using cryo-electron microscopy, we analyzed the structure of the complex, formed by RNA polymerase stalled at position +42 during its transcription through the nucleosome. We obtained both projection images and a three-dimensional structure of the EC-42 complex at 2.5 nm resolution. This allowed us to confirm the conformational integrity of the nucleosome during the passage of the RNA polymerase.
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Об авторах
O. Volokh
Department of Bioengineering, School of Biology
Email: sokolova@mail.bio.msu.ru
Россия, Moscow, 119234
F. Hsieh
Robert Wood Johnson Medical School
Email: sokolova@mail.bio.msu.ru
США, Paterson St. 125, New Brunswick, NJ, 08901
M. Karlova
Department of Bioengineering, School of Biology
Email: sokolova@mail.bio.msu.ru
Россия, Moscow, 119234
E. Trifonova
Department of Bioengineering, School of Biology
Email: sokolova@mail.bio.msu.ru
Россия, Moscow, 119234
V. Studitsky
Cancer Epigenetics Team
Email: sokolova@mail.bio.msu.ru
США, Cottman Avenue 333, Philadelphia, PA, 19111
O. Sokolova
Department of Bioengineering, School of Biology
Автор, ответственный за переписку.
Email: sokolova@mail.bio.msu.ru
Россия, Moscow, 119234
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