Secretion of Extracellular Proteinases Active against Fibrillar Proteins by Micromycetes


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Аннотация

It has been shown that micromycetes Aspergillus ustus 1 and Tolypocladium inflatum k1 secrete proteolytic enzymes that possess high collagenolytic, fibrinolytic, and elastolytic activity. The activity of proteinases hydrolyzing fibrillar proteins, which was determined by the cleavage of azo-collagen, was 122.6 × 10–3EAzc/mL in A. ustus 1 and 69.7 × 10–3EAzc/mL in T. inflatum k1 (EAzc is the amount of azocollagen cleaved in 1 min (μg). The maximum values of activity were observed during submerged cultivation of A. ustus 1 for 4 days and of T. inflatum k1 for 5 days. It has been shown that the maximum of collagenolytic and general proteolytic activity during the cultivation of A. ustus 1 are time-separated, unlike T. inflatum k1, which, presumably, can simplify the procedure for obtaining proteinases active against fibrillar proteins.

Авторлар туралы

E. Popova

Department of Biology

Email: aosmol@mail.ru
Ресей, Moscow, 119234

D. Bednenko

Department of Biology

Email: aosmol@mail.ru
Ресей, Moscow, 119234

A. Osmolovskiy

Department of Biology

Хат алмасуға жауапты Автор.
Email: aosmol@mail.ru
Ресей, Moscow, 119234

V. Kreyer

Department of Biology

Email: aosmol@mail.ru
Ресей, Moscow, 119234

I. Kotova

Department of Biology

Email: aosmol@mail.ru
Ресей, Moscow, 119234

N. Egorov

International Biotechnology Center

Email: aosmol@mail.ru
Ресей, Moscow, 119234

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