Email this article Analysis of Element Composition of DNA-Protein Crystals In Vitro
- Authors: Moiseenko A.V.1, Loiko N.G.2,3, Chertkov O.V.1, Feofanov A.V.1,4, Krupyanskii Y.F.3, Sokolova O.S.1
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Affiliations:
- Department of Biology, Moscow State University
- Research Center of Biotechnology, Russian Academy of Sciences
- Semenov Institute of Chemical Physics, Russian Academy of Sciences
- Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences
- Issue: Vol 74, No 4 (2019)
- Pages: 240-245
- Section: Research Article
- URL: https://journals.rcsi.science/0096-3925/article/view/173924
- DOI: https://doi.org/10.3103/S0096392519040102
- ID: 173924
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Abstract
The universal response of Escherichia coli to stress is enhancing the synthesis of specific histone-like Dps proteins that bind bacterial DNA. As a result, two-dimensional and three-dimensional crystalline arrays can be observed in the cytoplasm of starving bacteria. Conditions for obtaining in vitro co-crystals of DNA-Dps were selected, and their elemental composition was studied using analytical electron microscopy. It was found that Dps in the co-crystal retains its ferritin-like activity; that is, it can stimulate the oxidation of Fe2+ ions to Fe3+ and facilitate the accumulation of iron in the form of Fe2O3 in the inner cavity of the oligomer.
About the authors
A. V. Moiseenko
Department of Biology, Moscow State University
Email: sokolova@mail.bio.msu.ru
Russian Federation, Moscow, 119234
N. G. Loiko
Research Center of Biotechnology, Russian Academy of Sciences; Semenov Institute of Chemical Physics, Russian Academy of Sciences
Email: sokolova@mail.bio.msu.ru
Russian Federation, Moscow, 119071; Moscow, 117977
O. V. Chertkov
Department of Biology, Moscow State University
Email: sokolova@mail.bio.msu.ru
Russian Federation, Moscow, 119234
A. V. Feofanov
Department of Biology, Moscow State University; Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences
Email: sokolova@mail.bio.msu.ru
Russian Federation, Moscow, 119234; Moscow, 117997
Yu. F. Krupyanskii
Semenov Institute of Chemical Physics, Russian Academy of Sciences
Email: sokolova@mail.bio.msu.ru
Russian Federation, Moscow, 117977
O. S. Sokolova
Department of Biology, Moscow State University
Author for correspondence.
Email: sokolova@mail.bio.msu.ru
Russian Federation, Moscow, 119234
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