Photocurable Hydrogels Containing Spidroin or Fibroin


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Abstract

Photocurable biocompatible hydrogels were fabricated from methacrylated gelatin and silk proteins (a recombinant analogue of spidroin from Nephila clavipes spider web and fibroin from the cocoons of the silkworm Bombyx mori). These polymers are widely applicable in tissue engineering due to their biocompatibility and biodegradability. Hydrogels were fabricated using two different methods that allowed to obtain either the macroscopic scaffolds or microstructures with a defined shape. Three-dimensional hydrogel samples were prepared via monomers solution photopolymerization for 10 min. As a result, the disk-shaped samples of hydrogels approximately 13 mm in diameter were fabricated. Their porous structure was confirmed by scanning electron microscopy. Microstructures were formed on coverslips using an Eclipse Ti-E microscope equipped with an A1 confocal module (Nikon, Japan) and 405 nm laser. This approach allows to control the topographic features of the obtained substrates, and it is viable for creating the micropattern surfaces to reveal for studying the interaction of cells with a substrate.

About the authors

I. V. Bessonov

Department of Biology

Email: kotlyarova.ms@gmail.com
Russian Federation, Moscow, 119234

M. S. Kotliarova

Department of Biology

Author for correspondence.
Email: kotlyarova.ms@gmail.com
Russian Federation, Moscow, 119234

M. N. Kopitsyna

Department of Biology

Email: kotlyarova.ms@gmail.com
Russian Federation, Moscow, 119234

A. V. Fedulov

Vladimirsky Moscow Regional Scientific Research Clinical Institute

Email: kotlyarova.ms@gmail.com
Russian Federation, Moscow, 129110

A. M. Moysenovich

Department of Biology

Email: kotlyarova.ms@gmail.com
Russian Federation, Moscow, 119234

A. Yu. Arkhipova

Department of Biology

Email: kotlyarova.ms@gmail.com
Russian Federation, Moscow, 119234

V. G. Bogush

State Research Institute for Genetics and Selection of Industrial Microorganisms

Email: kotlyarova.ms@gmail.com
Russian Federation, Moscow, 117545

D. V. Bagrov

Department of Biology

Email: kotlyarova.ms@gmail.com
Russian Federation, Moscow, 119234

A. A. Ramonova

Department of Biology

Email: kotlyarova.ms@gmail.com
Russian Federation, Moscow, 119234

A. E. Mashkov

Vladimirsky Moscow Regional Scientific Research Clinical Institute

Email: kotlyarova.ms@gmail.com
Russian Federation, Moscow, 129110

K. V. Shaitan

Department of Biology

Email: kotlyarova.ms@gmail.com
Russian Federation, Moscow, 119234

M. M. Moisenovich

Department of Biology

Email: kotlyarova.ms@gmail.com
Russian Federation, Moscow, 119234

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