Photocurable Hydrogels Containing Spidroin or Fibroin
- Authors: Bessonov I.V.1, Kotliarova M.S.1, Kopitsyna M.N.1, Fedulov A.V.2, Moysenovich A.M.1, Arkhipova A.Y.1, Bogush V.G.3, Bagrov D.V.1, Ramonova A.A.1, Mashkov A.E.2, Shaitan K.V.1, Moisenovich M.M.1
-
Affiliations:
- Department of Biology
- Vladimirsky Moscow Regional Scientific Research Clinical Institute
- State Research Institute for Genetics and Selection of Industrial Microorganisms
- Issue: Vol 73, No 1 (2018)
- Pages: 24-27
- Section: Methods
- URL: https://journals.rcsi.science/0096-3925/article/view/173684
- DOI: https://doi.org/10.3103/S0096392518010030
- ID: 173684
Cite item
Abstract
Photocurable biocompatible hydrogels were fabricated from methacrylated gelatin and silk proteins (a recombinant analogue of spidroin from Nephila clavipes spider web and fibroin from the cocoons of the silkworm Bombyx mori). These polymers are widely applicable in tissue engineering due to their biocompatibility and biodegradability. Hydrogels were fabricated using two different methods that allowed to obtain either the macroscopic scaffolds or microstructures with a defined shape. Three-dimensional hydrogel samples were prepared via monomers solution photopolymerization for 10 min. As a result, the disk-shaped samples of hydrogels approximately 13 mm in diameter were fabricated. Their porous structure was confirmed by scanning electron microscopy. Microstructures were formed on coverslips using an Eclipse Ti-E microscope equipped with an A1 confocal module (Nikon, Japan) and 405 nm laser. This approach allows to control the topographic features of the obtained substrates, and it is viable for creating the micropattern surfaces to reveal for studying the interaction of cells with a substrate.
About the authors
I. V. Bessonov
Department of Biology
Email: kotlyarova.ms@gmail.com
Russian Federation, Moscow, 119234
M. S. Kotliarova
Department of Biology
Author for correspondence.
Email: kotlyarova.ms@gmail.com
Russian Federation, Moscow, 119234
M. N. Kopitsyna
Department of Biology
Email: kotlyarova.ms@gmail.com
Russian Federation, Moscow, 119234
A. V. Fedulov
Vladimirsky Moscow Regional Scientific Research Clinical Institute
Email: kotlyarova.ms@gmail.com
Russian Federation, Moscow, 129110
A. M. Moysenovich
Department of Biology
Email: kotlyarova.ms@gmail.com
Russian Federation, Moscow, 119234
A. Yu. Arkhipova
Department of Biology
Email: kotlyarova.ms@gmail.com
Russian Federation, Moscow, 119234
V. G. Bogush
State Research Institute for Genetics and Selection of Industrial Microorganisms
Email: kotlyarova.ms@gmail.com
Russian Federation, Moscow, 117545
D. V. Bagrov
Department of Biology
Email: kotlyarova.ms@gmail.com
Russian Federation, Moscow, 119234
A. A. Ramonova
Department of Biology
Email: kotlyarova.ms@gmail.com
Russian Federation, Moscow, 119234
A. E. Mashkov
Vladimirsky Moscow Regional Scientific Research Clinical Institute
Email: kotlyarova.ms@gmail.com
Russian Federation, Moscow, 129110
K. V. Shaitan
Department of Biology
Email: kotlyarova.ms@gmail.com
Russian Federation, Moscow, 119234
M. M. Moisenovich
Department of Biology
Email: kotlyarova.ms@gmail.com
Russian Federation, Moscow, 119234
Supplementary files
