Binase-Induced Changes of Tumor Cell Membranes
- Authors: Zelenikhin P.V.1, Gorbunova A.S.1, Beuerlein K.2, Makeeva A.V.1, Ilinskaya O.N.1
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Affiliations:
- Kazan Federal University
- Rudolf Buchheim Institute of Pharmacology
- Issue: Vol 73, No 1 (2018)
- Pages: 7-12
- Section: Cell Biology
- URL: https://journals.rcsi.science/0096-3925/article/view/173677
- DOI: https://doi.org/10.3103/S0096392518010091
- ID: 173677
Cite item
Abstract
Exogenous ribonucleases of Bacilli can selectively induce apoptosis of malignant cells. The ability of Bacillus pumilus ribonuclease, binase, to induce processes leading to a dynamic disruption of the integrity of A549 human pulmonary adenocarcinoma cell membranes was analyzed. The influence of different enzyme concentrations on the state of the cytoplasmic membrane of cells and mitochondrial membranes was characterized. Using the methods of flow cytofluorometry and fluorescence microscopy, it has been established that binase leads to disruption in normal functioning of both types of membranes, with mitochondrial membranes affected first. The study made it possible to identify and visualize the effects of binase on the membrane structures of target cells and to confirm that bacterial RNase induces apoptosis of target cells mainly through the “internal” (mitochondrial) pathway.
About the authors
P. V. Zelenikhin
Kazan Federal University
Email: avmakeeva.kpfu@gmail.com
Russian Federation, Kazan, 420008
A. S. Gorbunova
Kazan Federal University
Email: avmakeeva.kpfu@gmail.com
Russian Federation, Kazan, 420008
K. Beuerlein
Rudolf Buchheim Institute of Pharmacology
Email: avmakeeva.kpfu@gmail.com
Germany, Giessen, 35392
A. V. Makeeva
Kazan Federal University
Author for correspondence.
Email: avmakeeva.kpfu@gmail.com
Russian Federation, Kazan, 420008
O. N. Ilinskaya
Kazan Federal University
Email: avmakeeva.kpfu@gmail.com
Russian Federation, Kazan, 420008