Bioresorbable Scaffolds Based on Fibroin for Bone Tissue Regeneration
- Authors: Kotliarova M.S.1, Arkhipova A.Y.2, Moysenovich A.M.1, Kulikov D.A.3, Kulikov A.V.4, Kon’kov A.S.1, Bobrov M.A.3, Agapov I.I.5, Moisenovich M.M.3, Molochkov A.V.3, Goncharenko A.V.2, Shaitan K.V.1
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Affiliations:
- Chair of Bioengineering, Department of Biology
- Laboratory of Confocal Microscopy, Department of Biology
- Vladimirsky Moscow Regional Research and Clinical Institute (MONIKI)
- Institute of Theoretical and Experimental Biophysics
- Shumakov Federal Research Center of Transplantology and Artificial Organs
- Issue: Vol 72, No 4 (2017)
- Pages: 190-195
- Section: Methods
- URL: https://journals.rcsi.science/0096-3925/article/view/173652
- DOI: https://doi.org/10.3103/S0096392517040095
- ID: 173652
Cite item
Abstract
Using the tissue-engineered constructs based on scaffolds that imitate the extracellular matrix of living tissues unveils new opportunities in the treatment of various pathologies and injuries associated with tissue and organ damage. Silk fibroin of silkworm Bombyx mori is a biocompatible and bioresorbable polymer with high mechanical strength and elasticity that allows creating scaffolds on its basis for regeneration of various tissues, including bone. In the present work, fibroin scaffolds were obtained. They were designed in the form of porous sponges, films, and hybrid scaffolds of a bilayer structure in which the porous sponge threedimensional structure is limited on one side by a film. The structure of the scaffolds and their biocompatibility were studied: immortalized and primary fibroblasts, as well as the osteoblast-like cells, have been shown to successfully adhere and proliferate on the surface of the studied scaffolds. Numerous osteogenesis foci have been observed in the implant region 4 weeks after the fibroin porous scaffold implantation in the in vivo experiments in a rat femoral bone defect model indicating the osteoconduction of the scaffolds.
About the authors
M. S. Kotliarova
Chair of Bioengineering, Department of Biology
Author for correspondence.
Email: kotlyarova.ms@gmail.com
Russian Federation, Moscow, 119234
A. Yu. Arkhipova
Laboratory of Confocal Microscopy, Department of Biology
Email: kotlyarova.ms@gmail.com
Russian Federation, Moscow, 119234
A. M. Moysenovich
Chair of Bioengineering, Department of Biology
Email: kotlyarova.ms@gmail.com
Russian Federation, Moscow, 119234
D. A. Kulikov
Vladimirsky Moscow Regional Research and Clinical Institute (MONIKI)
Email: kotlyarova.ms@gmail.com
Russian Federation, Moscow, 129110
A. V. Kulikov
Institute of Theoretical and Experimental Biophysics
Email: kotlyarova.ms@gmail.com
Russian Federation, Pushchino, Moscow oblast, 142290
A. S. Kon’kov
Chair of Bioengineering, Department of Biology
Email: kotlyarova.ms@gmail.com
Russian Federation, Moscow, 119234
M. A. Bobrov
Vladimirsky Moscow Regional Research and Clinical Institute (MONIKI)
Email: kotlyarova.ms@gmail.com
Russian Federation, Moscow, 129110
I. I. Agapov
Shumakov Federal Research Center of Transplantology and Artificial Organs
Email: kotlyarova.ms@gmail.com
Russian Federation, Moscow, 113182
M. M. Moisenovich
Vladimirsky Moscow Regional Research and Clinical Institute (MONIKI)
Email: kotlyarova.ms@gmail.com
Russian Federation, Moscow, 129110
A. V. Molochkov
Vladimirsky Moscow Regional Research and Clinical Institute (MONIKI)
Email: kotlyarova.ms@gmail.com
Russian Federation, Moscow, 129110
A. V. Goncharenko
Laboratory of Confocal Microscopy, Department of Biology
Email: kotlyarova.ms@gmail.com
Russian Federation, Moscow, 119234
K. V. Shaitan
Chair of Bioengineering, Department of Biology
Email: kotlyarova.ms@gmail.com
Russian Federation, Moscow, 119234
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