Email this article Induction of osteogenic differentiation of osteoblast-like cells MG-63 during cultivation on fibroin microcarriers
- Authors: Kotliarova M.S.1, Zhuikov V.A.2, Chudinova Y.V.1,2, Khaidapova D.D.3, Moisenovich A.M.4, Kon’kov A.S.1, Safonova L.A.1,5, Bobrova M.M.1,5, Arkhipova A.Y.4, Goncharenko A.V.4, Shaitan K.V.1
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Affiliations:
- Department of Bioengineering, School of Biology
- Research Center of Biotechnology
- Department of Soil Physics and Reclamation, School of Soil Science
- Laboratory of Confocal Microscopy, School of Biology
- Shumakov Federal Research Center of Transplantology and Artificial Organs
- Issue: Vol 71, No 4 (2016)
- Pages: 212-217
- Section: Methods
- URL: https://journals.rcsi.science/0096-3925/article/view/173556
- DOI: https://doi.org/10.3103/S0096392516040052
- ID: 173556
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Abstract
We have developed microcarriers made from silk fibroin. Microcarriers can be used as a substrate for cell cultivation and cell delivery during cell-based therapy and for the construction of bioengineered tissue. Fibroin microcarriers were mineralized, which led to the appearance of calcium phosphate crystals on their surface. The ability of mineralized and nonmineralized microcarriers to support osteogenic differentiation of the osteoblast-like cell line MG-63 was estimated by alkaline phosphatase activity, an early marker of bone formation. The experiment showed cells actively proliferating on the surface of both mineralized and nonmodified microcarriers. Culturing MG-63 on the surface of fibroin microcarriers resulted in an increase of alkaline phosphatase activity indicative of osteogenic differentiation of MG-63 cells in the absence of inductors. The level of alkaline phosphatase was higher when mineralized microcarriers were used. Alkaline phosphatase activity of MG-63 cells cultivated using traditional two-dimensional approaches were close to zero. As opposed to conventional monolayer culturing, microcarrier culture cells are in a three-dimensional environment that is closer to physiological conditions. This can have a significant impact on their morphology and functional properties. During this study, we also characterized mechanical properties of porous scaffolds used for microcarriers.
About the authors
M. S. Kotliarova
Department of Bioengineering, School of Biology
Author for correspondence.
Email: kotlyarova.ms@gmail.com
Russian Federation, Moscow, 119234
V. A. Zhuikov
Research Center of Biotechnology
Email: kotlyarova.ms@gmail.com
Russian Federation, Moscow, 119071
Y. V. Chudinova
Department of Bioengineering, School of Biology; Research Center of Biotechnology
Email: kotlyarova.ms@gmail.com
Russian Federation, Moscow, 119234; Moscow, 119071
D. D. Khaidapova
Department of Soil Physics and Reclamation, School of Soil Science
Email: kotlyarova.ms@gmail.com
Russian Federation, Moscow, 119234
A. M. Moisenovich
Laboratory of Confocal Microscopy, School of Biology
Email: kotlyarova.ms@gmail.com
Russian Federation, Moscow, 119234
A. S. Kon’kov
Department of Bioengineering, School of Biology
Email: kotlyarova.ms@gmail.com
Russian Federation, Moscow, 119234
L. A. Safonova
Department of Bioengineering, School of Biology; Shumakov Federal Research Center of Transplantology and Artificial Organs
Email: kotlyarova.ms@gmail.com
Russian Federation, Moscow, 119234; Moscow, 113182
M. M. Bobrova
Department of Bioengineering, School of Biology; Shumakov Federal Research Center of Transplantology and Artificial Organs
Email: kotlyarova.ms@gmail.com
Russian Federation, Moscow, 119234; Moscow, 113182
A. Y. Arkhipova
Laboratory of Confocal Microscopy, School of Biology
Email: kotlyarova.ms@gmail.com
Russian Federation, Moscow, 119234
A. V. Goncharenko
Laboratory of Confocal Microscopy, School of Biology
Email: kotlyarova.ms@gmail.com
Russian Federation, Moscow, 119234
K. V. Shaitan
Department of Bioengineering, School of Biology
Email: kotlyarova.ms@gmail.com
Russian Federation, Moscow, 119234
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