Screening of the culture media with different concentrations of nutrients for cultivation of the microalgae associated with the invertebrates of the White Sea
- 作者: Lobakova E.S.1, Selyakh I.O.1, Semenova L.R.1, Chivkunova O.B.1, Shcherbakov P.N.1, Solovchenko A.E.1,2
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隶属关系:
- Department of Biology
- Timiryazev Institute of Plant Physiology
- 期: 卷 71, 编号 2 (2016)
- 页面: 102-107
- 栏目: Methods
- URL: https://journals.rcsi.science/0096-3925/article/view/173500
- DOI: https://doi.org/10.3103/S009639251602005X
- ID: 173500
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详细
The growth and biomass accumulation of three microalgal strains of Desmodesmus (Scenedesmaceae, Chlorophyceae), 1Рm66В, 2Cl66E, and 3Dp86Е-1, isolated from the White Sea benthic invertebrates were studied under conditions of batch culture in different standard media (BG-11, Prat, Goldberg, Gromov, Tamiya, artificial seawater) and modified media. The culture condition, biomass accumulation, and uptake of nitrate and phosphate were recorded. A significant alkalization of the culture medium up to pH 10 has been observed during a vigorous growth of the microalgae. The most significant biomass accumulation has been recorded in BG-11 (in complete or modified medium with addition of artificial seawater), Tamiya, and Prat media. Addition of seawater did not affect the growth of Desmodesmus sp. in the nitrate-containing media, although that maintained growth of the microalgae in the nitrogen-lacking media without cell aggregation. The BG-11 medium appears suitable for isolation and cultivation of both symbiotic and free-living microalgae by all the tested features. The Prat medium is the best for maintaining the microalgal strains in living collection.
作者简介
E. Lobakova
Department of Biology
编辑信件的主要联系方式.
Email: elena.lobakova@rambler.ru
俄罗斯联邦, Moscow, 119234
I. Selyakh
Department of Biology
Email: elena.lobakova@rambler.ru
俄罗斯联邦, Moscow, 119234
L. Semenova
Department of Biology
Email: elena.lobakova@rambler.ru
俄罗斯联邦, Moscow, 119234
O. Chivkunova
Department of Biology
Email: elena.lobakova@rambler.ru
俄罗斯联邦, Moscow, 119234
P. Shcherbakov
Department of Biology
Email: elena.lobakova@rambler.ru
俄罗斯联邦, Moscow, 119234
A. Solovchenko
Department of Biology; Timiryazev Institute of Plant Physiology
Email: elena.lobakova@rambler.ru
俄罗斯联邦, Moscow, 119234; ul. Botanicheskaya 35, Moscow, 127276
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