Occurrence of grapevine leafroll-associated viruses-1 and -3 in Crimea
- Authors: Volkov Y.A.1, Dmitrenko U.D.2, Volodin V.A.1, Gorislavets S.M.1, Stranishevskaya E.P.1, Agranovsky A.A.3, Kamionskaya A.M.2, Vinogradova S.V.2, Porotikova E.V.2, Risovannaya V.I.1
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Affiliations:
- Magarach All-Russia National Research Institute for Viticulture and Wine-Making
- Research Center of Biotechnology
- Department of Biology
- Issue: Vol 71, No 2 (2016)
- Pages: 76-79
- Section: Virology
- URL: https://journals.rcsi.science/0096-3925/article/view/173485
- DOI: https://doi.org/10.3103/S0096392516020097
- ID: 173485
Cite item
Abstract
Grapevine leafroll is one of the most widespread and harmful grapevine diseases. To investigate the occurrence of grapevine leafroll-associated viruses-1 and 3, the survey of vineyards has been conducted in 2015 in six regions of the Crimea. A total of 689 leaf samples with virus symptoms were collected. GLRaV-1 and GLRaV-3 were analyzed by RT-PCR with the specific primers, followed by sequencing of the PCR products. GLRaV-1 and GLRaV-3 were detected in 34 (4.9%) and 37 (5.4%) of the samples, respectively. Vineyards in Simferopol, Bakhchisarai, and Sevastopol regions were found to be free of GLRaV-1 and GLRaV-3.
About the authors
Y. A. Volkov
Magarach All-Russia National Research Institute for Viticulture and Wine-Making
Email: coatprotein@bk.ru
Russian Federation, ul. Kirova 31, Yalta, Republic of the Crimea, 298600
U. D. Dmitrenko
Research Center of Biotechnology
Email: coatprotein@bk.ru
Russian Federation, pr. Leninskii 33-2, Moscow, 119071
V. A. Volodin
Magarach All-Russia National Research Institute for Viticulture and Wine-Making
Email: coatprotein@bk.ru
Russian Federation, ul. Kirova 31, Yalta, Republic of the Crimea, 298600
S. M. Gorislavets
Magarach All-Russia National Research Institute for Viticulture and Wine-Making
Email: coatprotein@bk.ru
Russian Federation, ul. Kirova 31, Yalta, Republic of the Crimea, 298600
E. P. Stranishevskaya
Magarach All-Russia National Research Institute for Viticulture and Wine-Making
Email: coatprotein@bk.ru
Russian Federation, ul. Kirova 31, Yalta, Republic of the Crimea, 298600
A. A. Agranovsky
Department of Biology
Email: coatprotein@bk.ru
Russian Federation, Moscow, 119234
A. M. Kamionskaya
Research Center of Biotechnology
Email: coatprotein@bk.ru
Russian Federation, pr. Leninskii 33-2, Moscow, 119071
S. V. Vinogradova
Research Center of Biotechnology
Author for correspondence.
Email: coatprotein@bk.ru
Russian Federation, pr. Leninskii 33-2, Moscow, 119071
E. V. Porotikova
Research Center of Biotechnology
Email: coatprotein@bk.ru
Russian Federation, pr. Leninskii 33-2, Moscow, 119071
V. I. Risovannaya
Magarach All-Russia National Research Institute for Viticulture and Wine-Making
Email: coatprotein@bk.ru
Russian Federation, ul. Kirova 31, Yalta, Republic of the Crimea, 298600
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