Occurrence of grapevine leafroll-associated viruses-1 and -3 in Crimea


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Abstract

Grapevine leafroll is one of the most widespread and harmful grapevine diseases. To investigate the occurrence of grapevine leafroll-associated viruses-1 and 3, the survey of vineyards has been conducted in 2015 in six regions of the Crimea. A total of 689 leaf samples with virus symptoms were collected. GLRaV-1 and GLRaV-3 were analyzed by RT-PCR with the specific primers, followed by sequencing of the PCR products. GLRaV-1 and GLRaV-3 were detected in 34 (4.9%) and 37 (5.4%) of the samples, respectively. Vineyards in Simferopol, Bakhchisarai, and Sevastopol regions were found to be free of GLRaV-1 and GLRaV-3.

About the authors

Y. A. Volkov

Magarach All-Russia National Research Institute for Viticulture and Wine-Making

Email: coatprotein@bk.ru
Russian Federation, ul. Kirova 31, Yalta, Republic of the Crimea, 298600

U. D. Dmitrenko

Research Center of Biotechnology

Email: coatprotein@bk.ru
Russian Federation, pr. Leninskii 33-2, Moscow, 119071

V. A. Volodin

Magarach All-Russia National Research Institute for Viticulture and Wine-Making

Email: coatprotein@bk.ru
Russian Federation, ul. Kirova 31, Yalta, Republic of the Crimea, 298600

S. M. Gorislavets

Magarach All-Russia National Research Institute for Viticulture and Wine-Making

Email: coatprotein@bk.ru
Russian Federation, ul. Kirova 31, Yalta, Republic of the Crimea, 298600

E. P. Stranishevskaya

Magarach All-Russia National Research Institute for Viticulture and Wine-Making

Email: coatprotein@bk.ru
Russian Federation, ul. Kirova 31, Yalta, Republic of the Crimea, 298600

A. A. Agranovsky

Department of Biology

Email: coatprotein@bk.ru
Russian Federation, Moscow, 119234

A. M. Kamionskaya

Research Center of Biotechnology

Email: coatprotein@bk.ru
Russian Federation, pr. Leninskii 33-2, Moscow, 119071

S. V. Vinogradova

Research Center of Biotechnology

Author for correspondence.
Email: coatprotein@bk.ru
Russian Federation, pr. Leninskii 33-2, Moscow, 119071

E. V. Porotikova

Research Center of Biotechnology

Email: coatprotein@bk.ru
Russian Federation, pr. Leninskii 33-2, Moscow, 119071

V. I. Risovannaya

Magarach All-Russia National Research Institute for Viticulture and Wine-Making

Email: coatprotein@bk.ru
Russian Federation, ul. Kirova 31, Yalta, Republic of the Crimea, 298600

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