Methyl Jasmonate and Nitric Oxide in Regulation of the Stomatal Apparatus of Arabidopsis thaliana
- 作者: Yastreb T.1, Kolupaev Y.1,2, Kokorev A.1, Horielova E.1, Dmitriev A.3
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隶属关系:
- Dokuchaev Kharkiv National Agrarian University
- Karazin Kharkiv National University
- Institute of Cell Biology and Genetic Engineering, National Academy of Sciences of Ukraine
- 期: 卷 52, 编号 6 (2018)
- 页面: 400-405
- 栏目: Article
- URL: https://journals.rcsi.science/0095-4527/article/view/173988
- DOI: https://doi.org/10.3103/S0095452718060129
- ID: 173988
如何引用文章
详细
The effect of methyl jasmonate, donors and antagonists of nitric oxide (NO) on the state of stomata of Arabidopsis thaliana plants (Col-0) was studied. Treatment of the epidermis of leaves with a 50–400 μM solution of methyl jasmonate (MJ) caused a decrease in the size of the stomatal aperture and the percentage of open stomata. These effects were completely eliminated after preliminary treatment of epidermis cells with NO scavenger PTIO (2-phenyl-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide) and partially after treatment with inhibitors of animal NO synthase (NG-nitro-L-arginine methyl ester, L-NAME) and nitrate reductase (sodium tungstate). NO donors (L-arginine and sodium nitrite) in concentrations of 0.5–2.0 mM also caused a decrease in the number of open stomata and the size of the stomatal aperture. The action of L‑arginine on the state of stomata was leveled by pretreatment of cells with L-NAME, and effects of sodium nitrite were eliminated by preaction of sodium tungstate. A conclusion about the possible participation of nitrogen oxide, forming both as the result of L-arginine oxidation and during nitrate/nitrite reduction, in the regulation of the stomatal apparatus and about its role in the realization of an MJ effect on state of stomata in Arabidopsis thaliana was made.
作者简介
T. Yastreb
Dokuchaev Kharkiv National Agrarian University
编辑信件的主要联系方式.
Email: plant_biology@ukr.net
乌克兰, Kharkiv, 62483
Yu. Kolupaev
Dokuchaev Kharkiv National Agrarian University; Karazin Kharkiv National University
编辑信件的主要联系方式.
Email: plant_biology@ukr.net
乌克兰, Kharkiv, 62483; Kharkiv, 61022
A. Kokorev
Dokuchaev Kharkiv National Agrarian University
编辑信件的主要联系方式.
Email: plant_biology@ukr.net
乌克兰, Kharkiv, 62483
E. Horielova
Dokuchaev Kharkiv National Agrarian University
编辑信件的主要联系方式.
Email: plant_biology@ukr.net
乌克兰, Kharkiv, 62483
A. Dmitriev
Institute of Cell Biology and Genetic Engineering, National Academy of Sciences of Ukraine
编辑信件的主要联系方式.
Email: dmitriev.ap@gmail.com
乌克兰, Kyiv, 03143