Effect of substances with cryoprotective properties on surface marker CD44 in human erythrocytes
- Авторлар: Zemlianskykh N.1
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Мекемелер:
- Institute for Problems of Cryobiology and Cryomedicine
- Шығарылым: Том 50, № 3 (2016)
- Беттер: 203-213
- Бөлім: Article
- URL: https://journals.rcsi.science/0095-4527/article/view/173413
- DOI: https://doi.org/10.3103/S0095452716030117
- ID: 173413
Дәйексөз келтіру
Аннотация
The changes in surface marker CD44 in human erythrocytes exposed to cryoprotective media, as well as the impact of oxidative modification of membrane-cytoskeleton proteins on the CD44 characteristics under the changed physicochemical parameters of the cellular environment, were investigated in this study. Prolonged exposure of glycerol, DMSO, sucrose, and PEG-1500 caused a decrease in CD44 expression level and in amount of CD44-positive cells. That may reflect subtle rearrangements in the system of protein–protein interactions in the erythrocyte membrane-cytoskeleton complex, which may affect the stability of cells during cryopreservation. Extracellular substances (sucrose and PEG-1500) exhibited a more pronounced effect on the CD44 in erythrocytes in comparison with the examined substances of an intacellular type. Modification of membrane-cytoskeleton proteins with oxidizing bifunctional reagent diamide enhanced the identified tendencies.
Негізгі сөздер
Авторлар туралы
N. Zemlianskykh
Institute for Problems of Cryobiology and Cryomedicine
Хат алмасуға жауапты Автор.
Email: nzemliansky@gmail.com
Украина, Kharkiv