Studying Newly Synthesized and Developed 4-Hydroxy-3-Methoxybenzaldehyde Schiff Bases by UV Spectrophotometry and High Performance Liquid Chromatography
- 作者: Chigurupati S.1, Muralidharan S.1, Cin L.1, Raser W.1, Santhi K.2, Kesavanarayanan K.3
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隶属关系:
- Department of Pharmaceutical Chemistry, Faculty of Pharmacy, AIMST University
- Department of Pharmaceutical Technology, Faculty of Pharmacy, AIMST University
- Faculty of Pharmacy, University of Technology MARA (UiTM)
- 期: 卷 50, 编号 12 (2017)
- 页面: 851-856
- 栏目: Article
- URL: https://journals.rcsi.science/0091-150X/article/view/244491
- DOI: https://doi.org/10.1007/s11094-017-1544-0
- ID: 244491
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详细
Green synthesis is the design of chemical products and processes that reduces or eliminates the use and generation of hazardous substances. Schiff bases of 4-hydroxy-3- methoxybenzaldehyde (vanillin) were prepared using various aromatic amines and base catalyst in the presence of aqueous media. High performance liquid chromatography (HPLC) and ultraviolet (UV) spectrophotometry methods were developed for qualitative study of the synthesized 4-hydroxy-3- methoxybenzaldehyde Schiff bases. HPLC was carried out by reversed phase on a C18 column with a mobile phase composed of acetonitrile and 0.5% triethyl amine (pH 4.5 adjusted with orthophosphoric acid (60:40, v/v)). UV measurements were performed at λmax 286 nm for compound V1 ((E)-4-(4-hydroxy-3-methoxybenzylideneamino)benzoic acid), λmax 336 nm for compound V2 ((E)-4-(4-hydroxy-3-methoxybenzylideneamino)phenol), and λmax 320 nm for compound V3 ((2-methoxy-4-(E)-(phenylimino)methyl)phenol). Both the HPLC and UV methods showed good reproducibility and precision and were successfully applied to study 4-hydroxy-3- methoxybenzaldehyde derivatives. The standard de-viations of relative peak area and retention times for 4-hydroxy-3-methoxybenzaldehyde derivatives were within 2% indicating the suitability of the system. The proposed economical methods can be applied for rou-tine analysis of 4-hydroxy-3-methoxybenzaldehyde derivatives of any type.
作者简介
Sridevi Chigurupati
Department of Pharmaceutical Chemistry, Faculty of Pharmacy, AIMST University
编辑信件的主要联系方式.
Email: sridevi.phd@gmail.com
马来西亚, Semeling, Bedong, Kedah, 08100
Selvadurai Muralidharan
Department of Pharmaceutical Chemistry, Faculty of Pharmacy, AIMST University
Email: sridevi.phd@gmail.com
马来西亚, Semeling, Bedong, Kedah, 08100
Lim Cin
Department of Pharmaceutical Chemistry, Faculty of Pharmacy, AIMST University
Email: sridevi.phd@gmail.com
马来西亚, Semeling, Bedong, Kedah, 08100
Wei Raser
Department of Pharmaceutical Chemistry, Faculty of Pharmacy, AIMST University
Email: sridevi.phd@gmail.com
马来西亚, Semeling, Bedong, Kedah, 08100
Kumaraswamy Santhi
Department of Pharmaceutical Technology, Faculty of Pharmacy, AIMST University
Email: sridevi.phd@gmail.com
马来西亚, Semeling, Bedong, Kedah, 08100
Krishnan Kesavanarayanan
Faculty of Pharmacy, University of Technology MARA (UiTM)
Email: sridevi.phd@gmail.com
马来西亚, Puncak Alam, Selangor D. E., 42300