Efficacy of an Intravenous Form of Rifapentine in a Model of Experimental Tuberculosis in Mice
- Авторы: Ostrovskii K.1, Osipova N.1, Vanchugova L.1, Shipulo E.1, Potapov V.2, Pereverzeva É.3, Treshchalin I.3, Maksimenko O.1, Gel’perina S.1
-
Учреждения:
- Nanosistema Scientific Industrial Complex
- State Scientific Center for Applied Microbiology and Biotechnology
- G. F. Gauze Science Research Institute for the Discovery of New Antibiotics
- Выпуск: Том 51, № 7 (2017)
- Страницы: 616-621
- Раздел: Article
- URL: https://journals.rcsi.science/0091-150X/article/view/244732
- DOI: https://doi.org/10.1007/s11094-017-1663-7
- ID: 244732
Цитировать
Аннотация
Solubilization of rifapentine with human serum albumin (HSA) was used to produce a water-miscible form consisting of a colloidal suspension of particles of size 538 ± 9 nm. Dilution of the suspension more than 20-fold led to dissociation of the aggregates formed during solubilization procedure, producing a transparent solution. This was associated with a reduction in particle size to 10 – 20 nm, corresponding to the particle size in HSA solution at the same concentration. A fluorescence method showed that suspensions contained both free rifapentine and its complex with HSA. Studies of the activity against the pathogen of tuberculosis, Mycobacterium tuberculosis H37Rv, in a model of acute infection in Balb/c mice showed that the water-miscible form of rifapentine given intravenously had high activity against mycobacteria, comparable with the activity of rifapentine substance, decreasing mycobacterial loadings in the parenchymatous organs from 106 – 107 to 102 – 103 cfu/organ. Thus, use of HSA as solubilizer yielded an intravenous form of rifapentine retaining the activity of the antibiotic against Mycobacterium tuberculosis.
Ключевые слова
Об авторах
K. Ostrovskii
Nanosistema Scientific Industrial Complex
Автор, ответственный за переписку.
Email: ostrovkp@mail.ru
Россия, 20 Seventh Kozhukhovskaya Street, Moscow, 115193
N. Osipova
Nanosistema Scientific Industrial Complex
Email: ostrovkp@mail.ru
Россия, 20 Seventh Kozhukhovskaya Street, Moscow, 115193
L. Vanchugova
Nanosistema Scientific Industrial Complex
Email: ostrovkp@mail.ru
Россия, 20 Seventh Kozhukhovskaya Street, Moscow, 115193
E. Shipulo
Nanosistema Scientific Industrial Complex
Email: ostrovkp@mail.ru
Россия, 20 Seventh Kozhukhovskaya Street, Moscow, 115193
V. Potapov
State Scientific Center for Applied Microbiology and Biotechnology
Email: ostrovkp@mail.ru
Россия, Obolensk, Serpukhov District, Moscow Region, 142279
É. Pereverzeva
G. F. Gauze Science Research Institute for the Discovery of New Antibiotics
Email: ostrovkp@mail.ru
Россия, 11 Bol’shaya Pirogovskaya Street, Moscow, 119021
I. Treshchalin
G. F. Gauze Science Research Institute for the Discovery of New Antibiotics
Email: ostrovkp@mail.ru
Россия, 11 Bol’shaya Pirogovskaya Street, Moscow, 119021
O. Maksimenko
Nanosistema Scientific Industrial Complex
Email: ostrovkp@mail.ru
Россия, 20 Seventh Kozhukhovskaya Street, Moscow, 115193
S. Gel’perina
Nanosistema Scientific Industrial Complex
Email: ostrovkp@mail.ru
Россия, 20 Seventh Kozhukhovskaya Street, Moscow, 115193