Modern Methods of Analysis of Carotenoids (Review)

Abstract

Qualitative and quantitative methods of analysis of carotenoids that are used to analyze drugs, dietary supplements, and food products are reviewed. UV and visible spectrophotometry and chromatographic methods, e.g., thin-layer (TLC), high-performance liquid (HPLC), and supercritical fluid (SFC) chromatography, are discussed. The importance of using hybrid chromatography methods with IR, NMR, and mass-spectroscopic detectors is demonstrated. The dependence of carotenoid chromatographic separation parameters on sorbate polarity (dipole moment) in TLC, HPLC, and SFC is demonstrated for the first time.